DNA Sample #1
5ââ T C C A G T G A T C T C G A A T T C G C T A G T A A C G T T C G A T
3ââ A G G T C A C T A G A G C T T A A G C G A T C A T T G C A A G C T A
DNA Sample #2
5ââ T C A T C G A A T T C C T G G A A T C A G C A G A A T T C G C A T A
3ââ A G T A G C T T A A G G A C C T T A G T C G T C T T A A G C G T A T
If both samples are treated with the restriction enzyme EcoRI [recognition sequence GâAATTC] then indicate the number of fragments and the size of each fragment from each sample of DNA. List the fragment sizes in order of largest to smallest.
Sample # 1 Sample # 2
# of fragments:________ # of fragments:________
Sizes of fragments (large to small): Sizes of fragments (large to small):
DNA Sample #1
5ââ T C C A G T G A T C T C G A A T T C G C T A G T A A C G T T C G A T
3ââ A G G T C A C T A G A G C T T A A G C G A T C A T T G C A A G C T A
DNA Sample #2
5ââ T C A T C G A A T T C C T G G A A T C A G C A G A A T T C G C A T A
3ââ A G T A G C T T A A G G A C C T T A G T C G T C T T A A G C G T A T
If both samples are treated with the restriction enzyme EcoRI [recognition sequence GâAATTC] then indicate the number of fragments and the size of each fragment from each sample of DNA. List the fragment sizes in order of largest to smallest.
Sample # 1 Sample # 2
# of fragments:________ # of fragments:________
Sizes of fragments (large to small): Sizes of fragments (large to small):
Related textbook solutions
Related questions
DNA Structure and Function LabReport
- DNA Structure
- Which two scientists are credited with discovering DNA?
- Name the nitrogen bases that are purines.
- Which nitrogen base pairs with thymine?
- List the three components of a nucleotide.
- DNA Replication
- What is the purpose of DNA replication?
- How many times does replication occur in the life of acell?
- In the Lab, Exercise 2, the original strand on the left had thebases shown below. Input the new bases that correctly pair with theoriginal strand.
Original | New |
C | |
A | |
G | |
T |
- RNA Structure
- Describe the structure and function of RNA.
- Refer to Exercise 3 and record the bases of the RNA strandproduced from the replicated DNA strand.
DNA | RNA |
C | |
A | |
G | |
T |
- Record the differences between DNA and RNA in the tablebelow.
DNA | RNA | |
Sugars | ||
Bases | ||
Strands |
- RNA Synthesis
- The process of assembling RNA is called _________.
- How is replication different from transcription?
- Refer to Exercise 4. Write the letters for the base sequence ofmRNA in the spaces below DNA. Note that the order is reversed;start with the 3â end of the DNA strand and the 5â end of the mRNAstrand. Transcription is DNA to mRNA. Note RNA contains Uracilinstead of Thymine; There is no thymine in RNA.
DNA | 3â | C | G | T | C | G | T | C | C | A | A | T | T | 5â |
mRNA | 5â | 3â |
- Protein Synthesis
- What type of RNA provides amino acids to build polypeptidechains?
- If a mRNA strand has the bases 5â CUC 3â, what amino acid willbe translated? Refer to the printable chart in Exercise 5.
- Where in the cell does translation occur?
- Genes
- What could be the problem if there is a change in the basesequence of a gene as it is passed down to the offspring?
- Give an example of a disorder that results from changes in theamino acid sequence.
- What causes sickle cell anemia?
- Gene Cloning
- What is the function of a plasmid?
- Print the document from Lab, Exercise 6. Complete the activityalong with the video demonstration. Sign, date, and take an imageof your final product and include with this lab report.
