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28 Sep 2019
I need help with two questions regarding DNA sequencing...
1) Why might you find that your DNA isolation might havemultiple "perfect" matches (1.000) for more than one species? Giventhat 16S genes are used almost universally to discrimanate betweenspecies, How can you explain this result?
2) Why might you obtain a sequence trace that shows very strongsignal (tall, well-isolated peaks) but that nontheless has bothmajor and minor peaks at most nuceotide positions. What does thisresult indicate? Why might you fail to get any sequence matchscoring above 0.7000?
I need help with two questions regarding DNA sequencing...
1) Why might you find that your DNA isolation might havemultiple "perfect" matches (1.000) for more than one species? Giventhat 16S genes are used almost universally to discrimanate betweenspecies, How can you explain this result?
2) Why might you obtain a sequence trace that shows very strongsignal (tall, well-isolated peaks) but that nontheless has bothmajor and minor peaks at most nuceotide positions. What does thisresult indicate? Why might you fail to get any sequence matchscoring above 0.7000?
Patrina SchowalterLv2
28 Sep 2019
