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Where in the cell is the assembly of N-oligosaccharides on thesecretory and integral membrane proteins initiated? b. Brieflydescribe the mechanism of assembly and attachment of the coresegment of the carbohydrate chain to a typical targetpolypeptide.

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Hubert Koch
Hubert KochLv2
30 Sep 2019

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Related questions

12. You have undertaken a study ofprotein transport in live cultured eukaryotic cells, and yourobjective is to follow the in vivo movement of asecreted protein through the secretory pathway to the cellexterior. You have introduced a gene that encodes a mutant form ofthis protein tagged with Green Fluorescent Protein (GFP) into thecells. When the cells are grown at 42OC, the mutantprotein is folded in a way that causes it to be retained in therough ER, whereas at 32oC, the protein folds normallyand is able to proceed through the pathway. Briefly describe anexperiment that would allow you to accomplish your objective and besure to mention the microscopic technique you would use.

  1. Where in the cell is the assembly ofN-oligosaccharides on the secretory and integral membraneproteins initiated?
  2. b. Briefly describe the mechanism of assembly andattachment of the core segment of the carbohydrate chain to atypical target polypeptide.

33 Different glycoproteins can have a diverse array of oligosaccharides. Which of the statements below for making glycoproteins on plasma membrane is true?

(a) Extensive modification of oligosaccharides occurs in the extracellular space.

(b) Initial oligosaccharide chains are made by addition of one sugar at a time onto the protein in the ER lumen.

(c) Each layer of Golgi membrane structures contains identical modification enzymes for modifying sugar chain on proteins.

(d) ER lipids can be modified by sugar chain, which can be used for sugar chain modification of the proteins.

Answer true or false for the following

a. NSF unwinds the v-SNARE/t-SNARE complex to allow membrane fusion during docking

b. Clathrin forms “cages” on vesicles that originate from plasma membrane.

c. Clathrin binds to adaptor proteins that link clathrin to membrane proteins.

d. Clathrin vesicles are used for endocytosis – bringing material across the plasma membrane and often delivering it to the early endosome

e. Clathrin mediates the fusion of the vesicles with early endosomes

f. Coated pits are formed from not wearing antiperspirant.

g. Rab (small GTPases) bound with GTP is critical for proper tethering of a vesicle/target organelle pair

h. Clathrin exhibits quaternary structure, defined by the assembly of three heavy chains and three light chains to form a triskelion

i. Compare to other filaments, only intermediate filaments have intrinsic ability to assembly and require no nucleation or assembly proteins

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