Question

Enzymes Lab Report

1. Enzyme Structure and Function

1. Define the following:

1. enzyme

2. substrate

2. Explain how enzymes function.

3. Go to Lab, Section I, Exercise 1 to participate in an activitythat demonstrates the importance of enzymes. Record the colorintensity of the solution in each test tube at five and tenminutes.

Time (min)	Tube S1 Potato Extract + Catechol	Tube S2 Potato Extract + Water	Tube S3 Catechol + Water
0
5
10

1. How can benzoquinone be detected?

2. Why were potatoes used in the experiment?

2. Enzyme Specificity

1. Based on Section II, Exercise 2, as determined by colorintensity, to what extent does catechol oxidase react withhydroquinone?

2. What was the role of hydroquinone in the experiment?

3. Temperature and Enzyme Activity

1. Based on Lab, Section III, Exercise 3 record the relative colorintensity at five-minute intervals to determine the temperaturerange for catechol oxidase.

Time (min)	Tube 1 4oC	Tube 2 23oC	Tube 3 40oC	Tube 4 60oC	Tube 5 80oC	Tube 6 100oC
0
5
10

2. Construct a bar graph of the data for the ten-minute readings.Label axes and provide units. Sign, date and prepare an image ofyour graph and include it with this lab report.

3. What is the optimal temperature range for catechol oxidaseactivity?

4. pH and Enzyme Activity

1. Within what pH range will catechol oxidase catalyze catecholand oxygen to form benzoquinone?

2. Define pH.

3. What is the role of catechol in the experiment?

4. Which test tube contains the most acidic buffer solution?

5. Cofactors and Enzyme Activity

1. What is a cofactor?

2. What cofactor is necessary for catechol oxidase activity?

Summary Questions

1. What was the role of catechol oxidase in the experiments?

2. What was the purpose of developing a color intensityscale?

3. Identify three factors that influence the rate of enzymaticreactions.

4. Examine the data in Exercise 3 and suggest a hypothesisexplaining why people with a fever feel tired and listless.

5. People who have been suddenly submerged in cold water andremained for long periods of time have survived. Examine the datain Exercise 3 and explain how this is possible.

6. How does pH range affect the function and structure of catecholoxidase?

7. The stomach maintains a pH of approximately 3 to 5. Do allenzymes function at this pH level? Examine the data in Exercise 4and determine if catechol oxidase would function in the stomach.

8. What is enzyme specificity? Give an example.