Need help with these genetics questions please!!
1. A student created a mutation in the bli-4 gene and introducedthis gene sequence into C. elegans. The mutation in this genedisrupts the structure of the adult-stage cuticle, causing fluidfilled separations of the cuticle layers. The student thenengineered a bacteria to carry siRNA targeting the bli-4 gene in C.elegans, and fed the bacteria to the worms. After a week, thestudent noticed that instead of seeing adult worms with blisters,most adult worms were paralyzed. Suggest some reasons why he didnot see the blister mutation. (3 marks)
2. In a classical genetic analysis, scientists create mutationswithin or surrounding cloned gene sequences. Then, they introducethese mutated gene sequences into cell cultures or model organismsand examine the phenotypes. Geneticists are now adding RNA-inducedgene silencing to their gene analysis toolbox. Describe how youwould use RNA-induced gene silencing to study the role of theDrosophila tra gene in sex determination. Next, describe how youwould study tra function, using a classical genetic analysis. Whatare the advantages and limitations of each research approach?[Klug, W. S., Cummings, M. R., Spencer, C. A., & Palladino, M.A. (2015). Concepts of Genetics (11th ed.). Pearson.] (4 marks)
Need help with these genetics questions please!!
1. A student created a mutation in the bli-4 gene and introducedthis gene sequence into C. elegans. The mutation in this genedisrupts the structure of the adult-stage cuticle, causing fluidfilled separations of the cuticle layers. The student thenengineered a bacteria to carry siRNA targeting the bli-4 gene in C.elegans, and fed the bacteria to the worms. After a week, thestudent noticed that instead of seeing adult worms with blisters,most adult worms were paralyzed. Suggest some reasons why he didnot see the blister mutation. (3 marks)
2. In a classical genetic analysis, scientists create mutationswithin or surrounding cloned gene sequences. Then, they introducethese mutated gene sequences into cell cultures or model organismsand examine the phenotypes. Geneticists are now adding RNA-inducedgene silencing to their gene analysis toolbox. Describe how youwould use RNA-induced gene silencing to study the role of theDrosophila tra gene in sex determination. Next, describe how youwould study tra function, using a classical genetic analysis. Whatare the advantages and limitations of each research approach?[Klug, W. S., Cummings, M. R., Spencer, C. A., & Palladino, M.A. (2015). Concepts of Genetics (11th ed.). Pearson.] (4 marks)
