1. What is the Shine-Dalgarno sequence? What binds to the sequence during translation? Describe some of the ways in which the sequence can vary, addressing the consequences that any such variation has on translation efficiency. In Detail
2. Describe the A, P, and E sites of the ribosome. Where are they located? What occupies each of the sites during the following stages of translation:
a. upon creation of the 70s initiation complex
b. after an initial round of peptidyl transfer, prior to aminoacyl tRNA binding
c. following aminoacyl tRNA binding, but before the peptidyl transfer reaction
d. after peptidyl transfer, but before translocation is completed by EF-G
e. in the presence of puromycin, prior to peptidyl transfer
f. upon reaching a stop codon
g. following release of the polypeptide chain, but before the appearance of RRF
1. What is the Shine-Dalgarno sequence? What binds to the sequence during translation? Describe some of the ways in which the sequence can vary, addressing the consequences that any such variation has on translation efficiency. In Detail
2. Describe the A, P, and E sites of the ribosome. Where are they located? What occupies each of the sites during the following stages of translation:
a. upon creation of the 70s initiation complex
b. after an initial round of peptidyl transfer, prior to aminoacyl tRNA binding
c. following aminoacyl tRNA binding, but before the peptidyl transfer reaction
d. after peptidyl transfer, but before translocation is completed by EF-G
e. in the presence of puromycin, prior to peptidyl transfer
f. upon reaching a stop codon
g. following release of the polypeptide chain, but before the appearance of RRF
