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28 Sep 2019
Promoter Discussion Questions (2,1) Please help me Answer ALL to compare to my anwers and Thank you!
1) Saturation mutagenesis of a promoter region will occasionally identify a nucleotide in a positive promoter element that when mutated greatly increases transcription. What is the most likely explanation for why a mutation in a positive element would increase transcription?
4) Outline the roles of the TATA box in transcriptional initiation.
5) The TATA box is often described as the minimal RNA polymerase II promoter? Why is this description misleading.
6) Outline an S1 protection assay to determine the position of the 5â end of a mRNA?
Promoter Discussion Questions (2,1) Please help me Answer ALL to compare to my anwers and Thank you!
1) Saturation mutagenesis of a promoter region will occasionally identify a nucleotide in a positive promoter element that when mutated greatly increases transcription. What is the most likely explanation for why a mutation in a positive element would increase transcription?
4) Outline the roles of the TATA box in transcriptional initiation.
5) The TATA box is often described as the minimal RNA polymerase II promoter? Why is this description misleading.
6) Outline an S1 protection assay to determine the position of the 5â end of a mRNA?
Beverley SmithLv2
28 Sep 2019
