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Which of the following statements are true concerning amino acid activation?

Which of the following statements are true concerning amino acid activation?

1.Amino acid activation is ATP dependent

2.The amino acid is attached to the 3' end of the tRNA

3.There is one specific activating enzyme and at least one specific tRNA for each amino acid

4.Two separate enzymes are required, one to form the aminoacyl adenylate and another to attach the amino acid to the tRNA.

5.Only one type of Aminoacyl-synthetase is used

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Lelia Lubowitz
Lelia LubowitzLv2
28 Sep 2019
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Related questions

1. tRNA aminoacylation

a. What is aminoacylation? Where on the tRNA does it occur?

b. Why is ATP required for aminoacylation? Why is the hydrolysis of PPi generated during aminoacylation important?

c. What is the significance of the high-transfer potential of the ester bond created during tRNA aminoacylation?

d. Why is the specificity of aminoacyl-tRNA synthetases important? How do these enzymes ensure their specificity?

e. Are you familiar enough with amino acid side chains to answer questions about aminoacyl-tRNA synthetase specificity? (e.g. how might similar amino acids like valine, serine and threonine be distinguished?)

f. Do you understand why there are differences in amino acid recognition between activation and editing sites? And how those differences dictate the specificity of the two different activities?

Which of the following is FALSE?

A. the linkage of a tRNA to its correct amino acids is highly selective

B. the amino acid is covalently linked to the 3' end of the tRNA

C. there are two major classes of aminoacyl-tRNA synthetases

D. aminoacyl-tRNA synthetases link amino acids to tRNA without the need for an energy source

The energy for protein synthesis comes from which of the following?

a.

The ATP used in the synthesis of aminoacyl-AMP molecules that are then converted to aminoacyl-tRNAs
b.

The GTP that is bound to initiation factors used in the initiation and translocation phases of translation
c.

The high energy C-N bond of the amino acid
d.

a, b, and c
e.

a and b

The enzymatic process of "charging" a molecule of tRNA is similar to other enzyme catalyzed reactions. Which of the following correctly describes such similarities?

a.

Formation of an acyl-adenylate intermediate coupled to the hydrolysis of pyrophosphate in fatty acid activation is similar to reactions involved in the "charging" of tRNA.
b.

Proofreading and correcting abilities that prevent incorporation of the wrong molecule are analogous to the action of DNA polymerase.
c.

The aminoacyl-tRNA synthetase is a relatively non-specific enzyme (i.e., it can activate many different amino acids) and, in this respect, is similar to ribonucleotide reductase.
d.

a and b.

e.

b and c.

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