In the Meselson Stahl experiment, cells were grown for many generations in âheavyâ media, then grown for two generations in âlightâ media. If the double-stranded DNA is separated according to its density, what bands would you expect to see?
a. a light band and a medium band of equal size
b. a light, a heavy, and a medium band of equal size
c. a large medium band only
d. a light band and a heavy band, with the light band larger
e. a light band and a medium band, with the light band larger
In the Meselson Stahl experiment, cells were grown for many generations in âheavyâ media, then grown for two generations in âlightâ media. If the double-stranded DNA is separated according to its density, what bands would you expect to see?
| a. | a light band and a medium band of equal size | |
| b. | a light, a heavy, and a medium band of equal size | |
| c. | a large medium band only | |
| d. | a light band and a heavy band, with the light band larger | |
| e. a light band and a medium band, with the light band larger |
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One or more answers for the following questions! Need some help as soon as possible! Thanks!
In the Meselson/Stahl experiment, E. coli were first grown in media containing heavy nitrogen, 15N, and then transferred to light nitrogen, 14N at the beginning of the experiment.
What fraction of the DNA helices will consist of mixed DNA after 4 rounds of replication?
| Less than 25% | |
| More than 75% | |
| 25-50% | |
| 51-75% | |
| None |
Andrew Murray's sister, Andrea, is adding to her brother's work on chromosomes. She is using cells that are unable to synthesize adenine (âade) and histidine (âhis). The plasmid she is currently working with consists of an origin of replication and the Ade gene.
Following her transformation of the plasmid into her yeast, what media will the cells be plated on to select for cells that have picked up the plasmid?
| Media containing histidine | |
| Media containing adenine | |
| Media lacking adenine | |
| Media lacking histidine |
