1. In Gel electrophoresus of amplified PCR samples and stainging of agorse gels, why must the controls ( homozygous +/+, homozygous -/-, heterozygous +,-) be run through both PCR and gel electrophoresis steps?
a. Also according to the watson and crick papers about DNA, what is the novel feature of their model?
b. What comments do these authors make concering the relation of bases on one strand of the helix as compared to those on the others? How might this be suggestive of a possible copying mechanism for the genetic materal?
c.From these collective reports how many bases (residues) do the authors suggest occur in one turn of the helix?
d. Also, Watson and crick showed that a molecule of DNA can be broken length wise into linear halves by applying heat. What does this imply about the strength of the bons between the halfs of linear DNA? Are the bonds between the two halves likely to be stronger or weaker than the bonds that make up the sugar phosphate group?
1. In Gel electrophoresus of amplified PCR samples and stainging of agorse gels, why must the controls ( homozygous +/+, homozygous -/-, heterozygous +,-) be run through both PCR and gel electrophoresis steps?
a. Also according to the watson and crick papers about DNA, what is the novel feature of their model?
b. What comments do these authors make concering the relation of bases on one strand of the helix as compared to those on the others? How might this be suggestive of a possible copying mechanism for the genetic materal?
c.From these collective reports how many bases (residues) do the authors suggest occur in one turn of the helix?
d. Also, Watson and crick showed that a molecule of DNA can be broken length wise into linear halves by applying heat. What does this imply about the strength of the bons between the halfs of linear DNA? Are the bonds between the two halves likely to be stronger or weaker than the bonds that make up the sugar phosphate group?
