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28 Sep 2019
Consider a hypothetical phage whose DNA replicates exclusivelyby rolling circle replication. A phage with radioactive DNA in bothstrands infects a bacterium and is allowed to replicate in anonradioactive medium. Assume that only daughter DNA from theelongated branch ever gets packaged into progeny particles.
a. What fraction of the parental radioactivity will appear inthe progeny phage? b. How many progeny phage will containradioactive DNA? c. What is the fundamental difference between theinitiation of ΓΒΈ replication and that of the rolling circle?
Consider a hypothetical phage whose DNA replicates exclusivelyby rolling circle replication. A phage with radioactive DNA in bothstrands infects a bacterium and is allowed to replicate in anonradioactive medium. Assume that only daughter DNA from theelongated branch ever gets packaged into progeny particles.
a. What fraction of the parental radioactivity will appear inthe progeny phage? b. How many progeny phage will containradioactive DNA? c. What is the fundamental difference between theinitiation of ΓΒΈ replication and that of the rolling circle?
Sixta KovacekLv2
28 Sep 2019