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28 Sep 2019
You add 1000 active bacteria phage to a sensitive bacteriastrain. All penetrate/invade a bacterium, but before replicationstarts, you lyse the bacteria with chloroform (which doesn't hurtbacteriophage). How many active bacteriaphage do you expect torecover?
A. 0
B. 10
C. 100
D. 1000
E. 10,000
2. You serially dilute a stock of bacteriophage by sequentiallytaking: 10 ul into 1 ml, 10 ul into 1 ml, 100 ul into 0.9 ml andplating 0.1 ml on a sensitive strain of bacteria. You later count257 plaques on that plate. How many phage per ml were in theoriginal stock?
You add 1000 active bacteria phage to a sensitive bacteriastrain. All penetrate/invade a bacterium, but before replicationstarts, you lyse the bacteria with chloroform (which doesn't hurtbacteriophage). How many active bacteriaphage do you expect torecover?
A. 0
B. 10
C. 100
D. 1000
E. 10,000
2. You serially dilute a stock of bacteriophage by sequentiallytaking: 10 ul into 1 ml, 10 ul into 1 ml, 100 ul into 0.9 ml andplating 0.1 ml on a sensitive strain of bacteria. You later count257 plaques on that plate. How many phage per ml were in theoriginal stock?
Hubert KochLv2
28 Sep 2019
