Question

Part 1 - Cells
Specimen Number Organism Eukaryote or Prokaryote Where Organism isFound How Organism Obtains Nutrients
Spirogyra
Diatom
Paramecium
Lactobacillus
Euglena
Trypanosoma
Volvox
Radiolaria
Amoeba
Plant cells with nucleus (chloroplasts absent)

List two similarities between plant cells and animal cells

1.

2.

List two differences between plant and animal cells

1.

2.


Part 2 – Diffusion and Osmosis

Diffusion

Boiling water…



Cold water….



Osmosis

Label your 4 plastic cups A, B, C & D
Cut 4 small pieces of paper and using a pencil label them A, B, C& D. These labels will go inside your dialysis tubing.
Prepare your dialysis tubing as follows:
• Soak your dialysis tubing in distilled water until it isflexible
• Take a piece of dialysis tubing and tie a tight knot in oneend
• Open the other end of the tubing by rolling it between your thumband finger
• Fill bag A with 10mL of 1% sucrose solution. Insert label A andsqueeze out any air bubbles then tie a tight knot in the end of thetubing to seal it
• Check for leaks, blot dry and set aside
• Fill bag B with 10mL of 1% sucrose solution and completeprocedure as before
• Fill bag C with 10mL 25% sucrose solution and complete procedureas before
• Fill bag D with 10mL 50% sucrose solution and complete procedureas before
Make a visual assessment of each bag at time zero, i.e. beforeplacing it into the solution in the cup, and take notes.
Prepare the experimental setup as follows
• Fill cup A with 50% sucrose solution
• Fill cups B, C & D with 1% sucrose solution
• Add bag A to cup A; bag B to cup B and so on
Leave your experiment undisturbed for 60 minutes. Now carefullyremove each bag from the cup with its respective solution and blotdry. Measure the volume of liquid inside each bag using yourgraduated cylinder and record your data in the table below. Be sureto blot dry your graduated cylinder between measurements.
[2 pts]

Beginning volume (milliliters) End Volume (milliliters) Difference(milliliters)
Bag A 10
Bag B 10
Bag C 10
Bag D 10



1. Did water move into or out of all of the bags?




2. Which bags demonstrated osmosis? Draw a diagram to show watermovement in each of the bags.








3. Which bags showed the most rapid movement of water across themembrane? Explain your answer









4. Apply the information you have learned and briefly discuss whyit is important for animal cells and plant cells to have theability to balance water intake with water loss.



Part 3 – Cell Membranes

For all 6 treatments
Arrange your tubes in ascending intensity of red color. Determine ascale for yourself on a scale of 0 (no color) to 10 (darkest redcolor) and quantify the colors observed in your tubes. Record yourresults in the space below.

Tube Number Treatment Color Intensity (scale 0-10)
1 70oC
2 55oC
3 40oC
4 20oC
5 4oC (refrigerator)
6 -5oC (freezer)



1. Which treatment caused most membrane damage as reflected bycolor intensity?




2. Which treatment caused least membrane damage as reflected bycolor intensity? [2 pts]




3. In what way does extreme heat damage the membrane allowing thecontents to leak out of cells?







4. How does extreme cold damage the membrane allowing the contentsto leak out of cells?















5. Apply this knowledge to a situation that may arise in your dailylife or in nature and briefly explain your reasoning in the spacebelow.