Hello,
I am having a hard time understanding what temperature-sensitivemutation is about? I understand that the wild type phenotype isobserved at permissive temperature and the mutation can only beseen at the restrictive temperature. However no one is able toexplain to me what specific change in the amino acid sequenceaccounts for the temperature sensitive phenotype. What happens atthe molecular level?
I've been asked the following question during a recent test andI didnt even know how to answer it:
I have isolated a series of mutant strains. Below is adescription of the mutant phenotypes. Each mutant strain contains amutation in a single gene. Please describe what gene is defectiveand why.
A temperature sensitive strain of yeast grow slowly at thepermissive temperature of 30 C but does not grow at 37 C. At 30 C,proteins are synthesized but at a slower rate. However, at 37 Cprotein synthesis ceases after 30 minutes.
Hello,
I am having a hard time understanding what temperature-sensitivemutation is about? I understand that the wild type phenotype isobserved at permissive temperature and the mutation can only beseen at the restrictive temperature. However no one is able toexplain to me what specific change in the amino acid sequenceaccounts for the temperature sensitive phenotype. What happens atthe molecular level?
I've been asked the following question during a recent test andI didnt even know how to answer it:
I have isolated a series of mutant strains. Below is adescription of the mutant phenotypes. Each mutant strain contains amutation in a single gene. Please describe what gene is defectiveand why.
A temperature sensitive strain of yeast grow slowly at thepermissive temperature of 30 C but does not grow at 37 C. At 30 C,proteins are synthesized but at a slower rate. However, at 37 Cprotein synthesis ceases after 30 minutes.
