Question

Biochemists make stocksolutions of reagents and use them to make working concentrationsof buffers. Some of these include

A. 1 M Tris-HCl (pH7.6)

B. 1 MDithiothreitol?

C. 2 MKCl?

D. 1 MK2HPO4

E. 1 MKH2PO4?

F. 1M Potassium Phosphate(pH 7.2)

G. 10 M NaOH?

H. 1 MZnSO4?

I. 1 MMgCl2?

J. 0.5 MNa2EDTA.

Describe how to constructthese stock solutions. You decide on the final volumes and showyour calculations. Note: 1 M ZnSO4, 1 MMgCl2, and 1 M Dithiothreitol should not be greaterthan 50 ml because they are used sparingly.