1. How can we make anantibody to a specific protein?
2. In what ways can thisantibody be used as a molecular tool in studying thisprotein?
3. Explain the differencebetween an allosteric and competitive inhibitor.
4. What are the fourlevels of protein organization? Explain them.
5. In SDS-Page, proteinsare separated by length. If the same length proteins can fold intoloose (bigger) or condensed (smaller) shapes and can even complexwith other proteins, how are individual proteins separated solelyaccording to their length? Additionally, proteins can havedifferent charges. Proteins with more basic amino acids have agreater negative charge while proteins with more acidic amino acidshave a greater positive charge. Proteins travel from the negativeelectrode to positive electrode in SDS-Page. How does theirintrinsic charge not affect their migration (true for mostproteins)?
1. How can we make anantibody to a specific protein?
2. In what ways can thisantibody be used as a molecular tool in studying thisprotein?
3. Explain the differencebetween an allosteric and competitive inhibitor.
4. What are the fourlevels of protein organization? Explain them.
5. In SDS-Page, proteinsare separated by length. If the same length proteins can fold intoloose (bigger) or condensed (smaller) shapes and can even complexwith other proteins, how are individual proteins separated solelyaccording to their length? Additionally, proteins can havedifferent charges. Proteins with more basic amino acids have agreater negative charge while proteins with more acidic amino acidshave a greater positive charge. Proteins travel from the negativeelectrode to positive electrode in SDS-Page. How does theirintrinsic charge not affect their migration (true for mostproteins)?
