3. What allows chain termination to occur at each base of thetemplate during an automated sequencing reaction?
a. the presence of a relatively low concentration of ddNTPs
b. the presence of a relatively low concentration of dNTPs
c. the presence of a relatively high concentration of ddNTPs
d. the presence of a relatively high concentration of dNTPs
4. In automated fluorescent sequencing, what would happen if thefluorescently labeled ddATP was inadvertently omitted from thereaction?
a. DNA polymerase does not function without equal concentrationsof the four nucleotides
b there would be gaps in the sequence where thymines werepresent in the template DNA strand
c. the sequence would end where the first thymine wasencountered on the template DNA strand
d. the sequence would end where the first adenine should beincorporated
3. What allows chain termination to occur at each base of thetemplate during an automated sequencing reaction?
a. the presence of a relatively low concentration of ddNTPs
b. the presence of a relatively low concentration of dNTPs
c. the presence of a relatively high concentration of ddNTPs
d. the presence of a relatively high concentration of dNTPs
4. In automated fluorescent sequencing, what would happen if thefluorescently labeled ddATP was inadvertently omitted from thereaction?
a. DNA polymerase does not function without equal concentrationsof the four nucleotides
b there would be gaps in the sequence where thymines werepresent in the template DNA strand
c. the sequence would end where the first thymine wasencountered on the template DNA strand
d. the sequence would end where the first adenine should beincorporated
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