You have isolated C57BL6 embryonic stem cells and have tried anew culture condition to keep them pluripotent. To test for thesuccess of your culture protocol, you are injecting the cells intoa C57BL6 blastocyst. Once the pups are born, you notice that yourexperiment failed. Why?

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Beverley SmithLv2

28 Sep 2019

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Which of the following statements about pronuclear injection is accurate?

a. DNA is inserted into any specific cell type whose fate has already been determined, such as skin cells.

b. DNA is inserted into a single celled mouse zygote so that the insertion can be passed to most/all cells of the resulting mouse embryo.

c. DNA that is inserted can integrate randomly anywhere in the genome of the mouse.

d. DNA that is inserted integrates at a specific site in the genome via homologous recombination.

e. DNA that is inserted includes an antibiotic resistance marker to be able to select for the embryonic stem cells (growing in dishes) that have incorporated the new DNA.

f. both A and C

g. both B and C

To generate a gene knockout in mice, which of the following is required?

a. a method for screening to verify insertion into the target gene in the mouse chromosome.

b. regions of homology between the researcher-engineered gene and the target gene in the mouse chromosome.

c. mouse Embryonic stem (ES) cells, to increase the chance that many cells in the host mouse including germline cells (which will form sperm and eggs) will carry the change.

d. all of the above

You follow the scheme outlined in to generate a knockout of the mouse gene Myo6, which is involved in motor coordination and movement. You derive ES cells from WT (Agouti-coated) mice, and disrupt one copy of the Myo6 gene in these ES cells. You then you transplant the ES cells into a blastocyst from a black mouse to generate a chimera. As shown in the last step in the figure, you then cross the chimera to a homozygous black mouse.

Some chimeric animals do not produce offspring carrying the gene replacement, while other chimeric animals with the same gene replacement can easily produce offspring carrying the gene replacement. Why?

a. chimeric animals are unable to produce offspring.

b. the gene replacement prevents correct gamete formation.

c. some chimeras have a germline entirely composed of cells from the blastocyst rather than the modified cells whereas others have a germline entirely composed of modified cells rather than cells from the blastocyst.

When you cross the chimera with the black mouse, amongst 20 F1 progeny obtained, you see some Agouti mice and some Black mice. Which of the following statements about the F1 progeny is true?

a. all the F1 that are Agouti will be homozygous for the myo6 knockout allele.

b. all the F1 that are Agouti will be heterozygous for the myo6 knockout allele.

c. 1/2 of the F1 that are Agouti will be heterozygous for myo6 knockout allele.

d. all the F1 that are Black will be homozygous for the myo6 knockout allele.

A hot topic these days is the use of stem cells for biomedical research on potential cures and treatments for many deadly diseases. The best and most potent stem cells are removed from an early stage embryo. These embryos are in the 50-100 cell stage (or 5 days after conception) which is basically a ball of cells at this point (there are no identifiable human features or parts). These cells are removed and then treated with varying hormones, chemicals, etc to create specific types of cells that can be used in future research. These embryos are donated to research as they will not ever be used to have a baby and are simply thrown away.

Task: Do you support the use of embryonic stem cells in biomedical research? Why or why not? If so, do you believe embryos should be created for the sole use of harvesting stem cells? explain and write your sources

blackcoyote423

Pleaserephrase these sentences while maintaining the samemeaning:

1) Reproductive cloning

â¢ has been used to create Dolly the Sheep and many cloned cows,dogs, cats and horses.
â¢ If the low success rates and issues of safety could be improvedas discussed below, the technology can be used to mass-produceanimals with special qualities, such as animals that are importantagriculturally or are able to produce helpful drugs for humanuse
â¢ SCNT has environmental uses in that it can be used to repopulateendangered species, as has been shown with the wild ox and thegaur
â¢ Some supporters of human reproductive cloning also see it as away of overcoming male infertility, where other methods of assistedreproduction have failed.

2) THERAPEUTIC CLONING :It could help advance embryonic stem cellresearch.

â¢ The stem cells have to be able to be isolated from a source andthen grown in the laboratory
â¢ The stem cells have to be able to be turned into thespecific
cell type needed for treatment

3) DNA CLONING is essential to enable enough copies of a gene orDNA segment to be analysed for human genetic testing to diagnosegenetic conditions and enable predictive or presymptomatic genetictesting for genetic conditions in asymptomatic individuals orprenatally (see Genetics Fact Sheets 21 and 17C).It providesmedical benefits in the fields of therapeutic cloning andregenerative medicine.
â¢It provides great potential for discovering treatments and curesto a plethora of diseases including Parkinsonâs disease,schizophrenia, Alzheimerâs disease, Cancer, spinal cord injuries,diabetes and many more.
â¢Limbs and organs could be grown in a lab from stem cells and thenused in transplants or to help treat illnesses.
â¢It will help scientists to learn about human growth and celldevelopment.
â¢Scientists and doctors will be able to test millions of potentialdrugs and medicine, without the use of animals or humantesters.
An advantage of the usage of adult stem cells to treat disease isthat a patientâs own cells could be used to treat a patient. Riskswould be quite reduced because patientsâ bodies would not rejecttheir own cells.
â¢An advantage of using embryonic stem cells is that they candevelop into any cell types of the body, and may then be moreversatile than adult stem cells.
The Advantages of Cloning
ã
The fundamental criterion of medical science must be the defenceand promotion of the integral good of the human person, in keepingwith that unique dignity which is ours by virtue of ourhumanity.