Question

Hi, I really need up fiuring out how to do this. I'm doing a1:10 ratio dilution. I know it's regular chem. lab but I can'tremember. Equation and help please. Need by tonight.

Use 0.2 ml PCR tubes for these reactions. The thin wallfacilitates heat transfer. Be careful to not crush the tubesbetween your fingers. There is not much writing surface on thesetubes so it is best to create a log in your notebook and thennumber the tubes accordingly.

Reagent	Stock Conc	Final Conc	Dilution	Volume per Sample	Volume per Master Mix (N+1)
Template DNA				5 ul
5X GoTaq Flexi Buf	5X	1X
dNTP Mixture	10 mM	0.2 mM
MgCl2	25 mM	2.5 mM
D1S80 Fwd Primer	10 uM	0.5 uM
D1S80 Reverse Primer	10 uM	0.5 uM
GoTaq Hot Start Polymerase	5U/ul	1.25U
MGW (Molecular Grade Water)