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Microbiology question, please answer carefully, thanks!

Make a Table for our seven (7) Rogues Gallery Bacteria, giving the Plate Medium each is on this Week and giving the Source for each of these Bacteria. [A Table always requires a Title]

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Jamar Ferry
Jamar FerryLv2
28 Sep 2019

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Case Study:

Bacteria are everywhere—on our skin, in our food, suspended in our drinking water. Although the
microbial majority is benign, a few bacterial species can make us sick. To safeguard against food- and
water-borne disease, microbiologists routinely screen our drinking water for bacterial pathogens.
Animal feces contain coliform bacteria, microorganisms that inhabit the intestines of warm-blooded
animals. Many coliform bacteria are also found on plants and in soil and water. Coliform bacteria are
not pathogens themselves, but their presence indicates the possibility of finding pathogens. In contrast,
fecal coliform bacteria such as Escherichia coli are found in feces, and their presence in drinking water
indicates fecal contamination. E. coli can also be a pathogen itself, so if E. coli is found in drinking
water there is a good chance that other pathogens are present, too.1
To detect E. coli and other coliform bacteria, microbiologists filter water samples and place the filter in
a Petri dish containing growth medium such as Endo agar. Microorganisms from the water grow and
form colonies, giving an estimate of the number of bacteria in each milliliter of water.
While E. coli forms colored, shiny colonies on Endo agar, other coliforms grow as white or clear
colonies.

Dark colonies = E. coli
White/clear colonies = coliform bacteria
White + Dark colonies = total coliform
bacteria.

The guidelines of maximum allowable limits for total coliforms in drinking and recreational waters vary
by state. Representative limits appear in the table below.2, 3, 4

Table 1. Maximum allowable limits of fecal coliform bacteria
(cells/100 ml)
Total Coliforms E. Coli
Drinking Water 0 0
Recreational Waters 400 235

Questions
1. Why would we want to count the number of fecal coliforms in a water sample?
2. Do 400 coliforms per 100 milliliters strike you as very many?
3. How many other types of bacteria might be present in a normal water sample?
4. What problems might Mary encounter when she uses these techniques in Antarctica?

Question 19 (1 point)

Each standard sized petri plate (the ones that you use) holds upto .........ml of growth medium:

Question 19 options:

5 ml

25 ml

100 ml

40 ml

Save

Question 21 (1 point)

It is important to subculture your bacteria because:

Question 21 options:

To prevent contamination

To provide bacteria with fresh set of nutrients

Both of these

Save

Question 22 (1 point)

Which of the following methods would help to isolate yellow and white bacteria from a mixture:

Question 22 options:

Dip a sterile cotton swab in bacterial culture broth, and spread it throughout a TSA plate to create a bacterial lawn.

Procure inoculum from a pure bacterial culture growing on TSA slant, and spread the bacteria onto a TSA plate.

Procure bacterial inoculum using a sterilized inoculating loop and streak it into 4 quadrants of a TSA plate, sterilizing the inoculating loop in-between each quadrant.

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