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What is the difference between DNA methylation and histonemethylation? I understand that both include the repression of genesand that DNA methylation occurs at CpG islands. But when doeshistone methylation occur and not DNA? VIce Versa? Please includeRITS/RISC complexes in the answer as that is involved.

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Irving Heathcote
Irving HeathcoteLv2
28 Sep 2019
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Related questions

ESSAY QUESTION: Explain chromatin remodeling: what it is(provide a definition), what it does to DNA, and how chromatinchanges can be inherited by daughter cells. Underline the followingterms in your answer: histone tails, promoter, DNA replication,methylation. Make sure that your answer is complete and cohesive,and that relationships between chromatin structure and DNAavailability are clearly explained. Include 1 figure. You arerequired to illustrate how chromatin changes can be transferredfrom DNA ahead of a replication fork (i.e., not yet replicated DNA)to the DNA behind the fork.

Is there such thing as partial methylation of recognition sites for restriction enzymes. For example, a 6 kb plasmid is grown in two different strains. Then the plasmids are taken out and analyzed by gel electrophoresis. For strain 1 there is only a 3kb band, while for strain 2 there is a 3kb, 2kb, and 1kb band. What would be the explanation for strain 1 and why the restriction enzyme did not cut the same site as in strain 2. I thought methylation was supposed to occur at all restriction sites, but is there such thing as partial methylation? Or is there another reason for the outcome of strain 1?

Chapter 10

1.Outline the history of our knowledge on DNA up to Watson and Crick. What were the main contributions made by each researcher’s key experiment?

2.Explain the setup of the Hershey and Chase experiment, what would the results have been if protein was the genetic material?

3.Draw the structure of a DNA nucleotide, labeling each main component correctly. How does an RNA nucleotide differ?

4.If a section of double stranded DNA contains 19% Adenine, how much Thymine is present?

5.You are a researcher studying the genetic basis of heart attacks and have been working to determine the expression levels of different genes that might contribute to cancer formation. You obtain the DNA methylation status of five genes of interest (the data are shown in the table below). The plus (+) sign indicates the level of DNA methylation; more plus signs correlates with increased methylation levels.Based on this information which genes would you predict to have the highest rate of transcription?

Gene

Methylation levels

1

++

2

+++++

3

+++

4

++

5

+

What are the characteristics of the 3 main DNA forms?

Chapter 11

What are the different types of chromatin?

What are the structures and important roles for telomeres and centromeres?

What are the differences found between eukaryotic chromosomes and mitochondrial?

Chapter 12

Explain each of the different models of replication.

If you grow a culture of bacteria in media with radioactive nucleotides so that all DNA in the cells include radioactive nucleotides and then place the bacteria in new non radioactive media. After two rounds of replication what proportion of the DNA molecules will contain radioactivity?

Summarize the similarities and differences between rolling-circle replication, theta replication and linear eukaryotic replication.

What are the functions of the different DNA polymerases found in eukaryotic cells?

Draw a replication fork and include all key components and orientations. (Leading/lagging strands, DNA helicase, RNA primer and DNA gyrase)

What is the Holliday model of recombination and what are the necessary steps?

Chapter 13

What are the different types of RNA and what roles do they play?

Describe the properties and functions of each of the RNA polymerases and how they differ depending on the organism.

Describe in detail the process and mechanisms of transcription in both prokaryotes and eukaryotes.

Chapter 14

What are the primary purposes of each of the three post transcriptional modifications that occur in eukaryotic cells.

What is alternative splicing and what role does it play in the cell?

How is ribosomal RNA processed after transcription?

How do siRNA and miRNA work, describe/draw out the process in detail.

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