1
answer
0
watching
122
views
28 Sep 2019
In B. taurulious a few amino acids are removed fromthe C-terminus of the ? -globin enzyme after it is synthesized.
The sequence of the original C-terminus can bededuced by comparing it to a mutant in which the reading frame
is shifted by the insertion or deletion of anucleotide and the mutant ? -globin escapes cleavage. The aminoacid
sequences of the purified wild-type enzyme and theframe shift mutant from amino acid 263 to the C-terminal end
are given below:
Wild type: N M N G K
Frameshift mutant: N M I W Q I C V M K D
a- what was the mutational event that gave rise tothe frameshift mutant?
b- Deduce the number of amino acids in thesynthesized form of the wild-type enzyme and, as far as
possible, the sequence of the deleted C-terminus.
In B. taurulious a few amino acids are removed fromthe C-terminus of the ? -globin enzyme after it is synthesized.
The sequence of the original C-terminus can bededuced by comparing it to a mutant in which the reading frame
is shifted by the insertion or deletion of anucleotide and the mutant ? -globin escapes cleavage. The aminoacid
sequences of the purified wild-type enzyme and theframe shift mutant from amino acid 263 to the C-terminal end
are given below:
Wild type: N M N G K
Frameshift mutant: N M I W Q I C V M K D
a- what was the mutational event that gave rise tothe frameshift mutant?
b- Deduce the number of amino acids in thesynthesized form of the wild-type enzyme and, as far as
possible, the sequence of the deleted C-terminus.
Casey DurganLv2
28 Sep 2019
