Which of the following are ingredients for PCR of a cancer gene?
Buffer (Mg++, etc.)
Oligonucleotide primers
Template/target DNA
dNTP (dATP, dCTP, dGTP, dTTP)
Cyclic AMP
Thermostable DNA polymerase
Uracil (U)
Which of the following are ingredients for PCR of a cancer gene?
| Buffer (Mg++, etc.) | ||
| Oligonucleotide primers | ||
| Template/target DNA | ||
| dNTP (dATP, dCTP, dGTP, dTTP) | ||
| Cyclic AMP | ||
| Thermostable DNA polymerase | ||
| Uracil (U) |
Related textbook solutions
Related questions
Gene ExpressionQuestions
Question 1
#1) Match the following reactions to the correct step of proteinsynthesis by ribosomes.
___ Hydrolysis of GTP and disassembly of the translationcomplex.
___ binding of initiator aminoacyl-tRNA
___ formation of the peptide bond
___ translocation of ribosome
___ Use of 2 mole-equivalents of ATP and attachment of an aminoacid to a transfer RNA molecule
Answer Choices for Number #1
A. initiation step
B. elongation step
C. termination step
D. aminoacyl-tRNA synthetase reaction
Question 2
#2) Mark which of the following are needed for the synthesis ofRNA by RNA polymerase:
___ primer
___ ATP, CTP, GTP, UTP
___ dATP, dCTP, dGTP, dTTP
___ template
Question 3
#3) Mark which of the following are properties of a bacteria(prokaryotic) messenger RNA:
| ___ | It has a cap at the 5'-end. | |
| ___ | It has a poly-A tail at the 3'-end. | |
| ___ | It has a 5'-untranslated region (UTR). | |
| ___ | It has a 3'-untranslated (UTR) region. |
Question 4
#4) Match the following RNA molecules to their respectivefunctions in the cell.
___ transfer RNA
___ ribosomal RNA
___ messenger RNA
___ small nuclear RNA (snRNA)
Answer Choices for Number #4
A. This RNA serves as the template for protein synthesis.
B. This molecule is a molecular component of ribosomes.
C. This molecule is a component of spliceosomes and serves torecognize the exon-intron junctions
D. This RNA molecule is delivers the amino acid to the ribosome andit recognizes the appropriate codons with its anticodon loop.
