Question

PLEASE ANSWER ALL PARTS FULLY!

How was liquid hybridization used to compare species relatedness? What is the purpose/significance of Driver versus Tracer DNA? What is Subtractive Hybridization, how/why would you use this technique?

Compare Maxam-Gilbert and Sanger DNA sequencing methods. What are the more descriptive names for these techniques? How are the techniques similar? different? What reagents are used in each reaction? How are the results read? Do you get the sequence of the molecule you started with or the complementary molecule? How does fluorescent cycle sequencing differ from the original Sanger Method?