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. What is an mRNA?

2. How does the RT-PCR tell you if a gene is expressed in a particular cell type?

3. Where can RNases be found? How can you prevent RNase contamination in your samples during experiments involving RNA?

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Lelia Lubowitz
Lelia LubowitzLv2
28 Sep 2019

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Related questions

You want to find out the expression of a gene you think is involved in cancer. You perform RT-PCR, which reverse transcribes RNA into DNA and amplifies that DNA. How does this experiment help you learn about the expression of the gene?

[a] If you can detect the amount of RNA, you can detect expression of a gene

[b] RT-PCR can be used for only cancer cells and can therefore detect expression

[c] RT-PCR uses topoisomerase to amplify genes to detect their expression

[d] Cancer is expressed in many tissues and needs to be detected

1.How is QPCR able to quantify the amount of template copies in a patient sample? Contrast to End Point PCR?

2. What are the necessary components for a PCR reaction?

3. How can you tell if there is contamination of your PCR samples?

Hello can you help me with the following questions:

1. Which part of an udder was transferred to an empty oocyte to clone the sheep Dolly?

a. blastocyst (I think its this one)

b. meiotic spindle

c. intact udder cell

d. nucleus

2. What is the principle of nuclear equivalence?

a. Differentiated cells have lost DNA that codes for other cell types.

b. All nuclei are the same shape.

c. Only nuclei of germ cells contain DNA capable of producing an entire organism.

d. The genome of every somatic cell contains information capable of generating any other cell type in the body.

3. A gene contains 6 exons, numbered 1-6. Which of the following mRNA isoforms is not a possible transcriptional product of this gene?

a. 1-2-3-4-5-6

b. 2-5-3-4

c. 2-4-5-6

d. 5-6

4. Which of the following is a mechanism used to alter gene expression using mRNA transcripts?

a. Different stability (longevity) of mRNA transcripts

b. Inhibition of translation of certain mRNAs

c. Production of small RNA molecules (microRNAs) that block translation

d. All of the above

e. None of the above

5. You have recently started working in an embryology lab and have obtained a large number of unfertilized zebrafish oocytes. You decide to treat the oocytes with RNAse (a protein that will degrade RNAs, but leave DNA proteins intact) prior to fertilizing them. Will the resulting embryos develop into normal fish?

a. Yes, because RNAs from sperm cells can replace those lost from the egg.

b. Yes, because intact DNA is the only factor required for development to proceed.

c. No, because RNAse treatment will damage the structure of the oocyte.

d. No, because maternal mRNAs are required for the early stages of development.

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