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A fragment of a wild-type polypeptide is sequenced for sevenamino acids. The same polypeptide region is sequenced in fourmutants.

Wild-type N-Thr-His-Ser-Gly-Leu-Lys-Ala-C

Mutant 1 N-Thr-His-Ser-Val-Leu-Lys-Ala-C

Mutant 2 N-Thr-His-Ser-C

Mutant 3 N-Thr-Thr-Leu-Asp-C

Mutant 4 N-Thr-Gln-Leu-Trp-Ile-Glu-Gly-C

a. Use the available information to characterize each mutantusing the appropriate terms from this list: missense, nonsense,silent, frame-shift, transition, transversion.

b. Determine the wild-type mRNA sequence.

c. Identify the mutation that produces each mutantpolypeptide.

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Hubert Koch
Hubert KochLv2
28 Sep 2019
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Related questions

For each mutant sequences below, give the type of mutation that occurred to produce the mutant sequence. (Note: the “…” indicates unspecified amino acids and types of mutations you might want to consider are insertions, deletions, missense, nonsense, and silent).

Normal: Met-Gly-Glu-Thr-Lys-Val-Val- ... -Pro

Mutant 1: Met-Gly

Mutant 2: Met-Gly-Glu-Asp

Mutant 3: Met-Gly-Arg-Leu-Lys

Mutant 4: Met-Arg-Glu-Thr-Lys -Val-Val - ... -Pro

For the following strand of DNA, use the table below to provide: bases for the complementary mRNA and the associated amino acids. What do you notice about the genetic code for proline in the peptide? How would the loss of the first G or switching that G to a T affect the structure and function of this protein?

DNA: GGC CTA CAA GCC GGA ACA GTT ACC GCG TAA CGA

RNA: ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ???

Amino Acid:??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ???
(3 Letters)

Genetic Code Table (mRNA)

1st position
(5' end)

2nd position (middle)

 3rd position
(3' end)
U C A G
U Phe F
Phe F
Leu L
Leu L 		Ser S
Ser S
Ser S
Ser S		 Tyr Y
Tyr Y
STOP
STOP		 Cys C
Cys C
STOP
Trp W 		U
C
A
G
C Leu L
Leu L
Leu L
Leu L		 Pro P
Pro P
Pro P
Pro P		 His H
His H
Gln Q
Gln Q		 Arg R
Arg R
Arg R
Arg R		 U
C
A
G
A Ile I
Ile I
Ile I
Met M 		Thr T
Thr T
Thr T
Thr T		 Asn N
Asn N
Lys K
Lys K		 Ser S
Ser S
Arg R
Arg R		 U
C
A
G
G Val V
Val V
Val V
Val V		 Ala A
Ala A
Ala A
Ala A		 Asp D
Asp D
Glu E
Glu E		 Gly G
Gly G
Gly G
Gly G		 U
C
A
G

1. The amino acid sequence of a particular polypeptide in awild-type bacterium and three single point mutant strains are shownin the following table:

Wild type= Met-Tyr-Ile-Thr-Trp-Glu-Glu-Pro-Val-Lys

Mutant 1= Met-Tyr-Ile-Thr-Trp-Asp-Glu-Pro-Val-Lys

Mutant 2= Met-Tyr-Ile-Pro-Gly-Arg-Asn-Gln

Mutant 3= Met-Tyr-His-Tyr-Leu-Gly-Gly-Thr-Ser-Lys

  1. For each mutant indicate the type of point mutations(transition, transversion, insertion or deletion) and the codon inwhich it occurred.
  2. B. Determine to the extent that is possible, the ribonucleotidesequence of the wild type mRNA molecule.

2. Mutations in several different genes that encode enzymes inthe pathway for the synthesis of the heme group may cause a geneticdisorder called methemoglobinemia. The symptoms of this disorderrange form mild to death in the most severe cases. The enzymecytochrome b5 reductase is one of the enzymes in this pathway. Thisenzyme is responsible for reducing the iron in the heme group tothe ferrous state so that it can bind oxygen. Kobayashi et al.reviewed several mutations in this gene. One of these mutationscauses a Ser to Pro substitution. Explain what nucleotide changeand in which position in the codon can cause this amino acidchange. Assign all possible appropriate terms to this mutation.

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