Lab report 3: Cell storage, cell migration assay andgene manipulation
This section includes 1) Cell storage process; 2) Study cellmigration using CFA; 3) gene transfection.
Purpose and principle of eachexperiment:
Cell storage:
Colony Forming Assay
DNA transfection:
Methods and protocols of eachexperiment:
Cell storage: list thetemperature of from high to low during cell storage process. Writedown the step-wise protocol for cryopreservation:
CFA approach:
Protocols for DNA transfection incells:
Results and discussion:
Why are cells maintained at lowtemperature? What can cause cell death during storage (discussthose factors involved)? Why adopt a gradual temperature strategy?How to improve cell storage quality? Give example where cellstorage is applied.
What are your results of the CFA?Give examples where CFA technique can be applied.
What are the results of yourtransfection? What is the ratio of your transfection? How toimprove transfection ratio? (cell lines, DNA, lipofectamine etc).What are the best conditions for transfection?
Discuss how to achieve geneoverexpression, down-regulate gene function and totally disruptgene function (what technique you need to use). Discussthe properties of vectors.
What is the application of genemanipulation and what can be done with this?
Lab report 3: Cell storage, cell migration assay andgene manipulation
This section includes 1) Cell storage process; 2) Study cellmigration using CFA; 3) gene transfection.
Purpose and principle of eachexperiment:
Cell storage:
Colony Forming Assay
DNA transfection:
Methods and protocols of eachexperiment:
Cell storage: list thetemperature of from high to low during cell storage process. Writedown the step-wise protocol for cryopreservation:
CFA approach:
Protocols for DNA transfection incells:
Results and discussion:
Why are cells maintained at lowtemperature? What can cause cell death during storage (discussthose factors involved)? Why adopt a gradual temperature strategy?How to improve cell storage quality? Give example where cellstorage is applied.
What are your results of the CFA?Give examples where CFA technique can be applied.
What are the results of yourtransfection? What is the ratio of your transfection? How toimprove transfection ratio? (cell lines, DNA, lipofectamine etc).What are the best conditions for transfection?
Discuss how to achieve geneoverexpression, down-regulate gene function and totally disruptgene function (what technique you need to use). Discussthe properties of vectors.
What is the application of genemanipulation and what can be done with this?
