Question

9. You use reverse transcriptase and PCR to amplify the mRNA ofa tumor cell and its normal counterpart. You label the tumor cDNAswith rhodamine, which glows red, and the normal cDNAs withfluoresceine, which glows green. Match the result with theappropriate interpretation of which gene is in the spot.

I. spot glows red

II. spot glows green

III. spot glows yellow

IV. spot doesn’t glow	I	II	III IV
a	normal only	tumor only	both sources	neither source
b	tumor only	normal only	both sources	neither source
c	tumor only	normal only	neither source	both sources
d	tumor only	neither source	normal only	both sources
e	neither source	both sources	tumor only	normal only