🏷️ LIMITED TIME OFFER: GET 20% OFF GRADE+ YEARLY SUBSCRIPTION →
Log in
Biology
1
answer
0
watching
45
views

Vector unpacking is generally assumed to be necessary for DNA release and gene expression, but the extent to which dissociation affects gene delivery is unclear.

detail . what is "Vector unpacking "??????

Watch
1
answer
0
watching
45
views

For unlimited access to Homework Help, a Homework+ subscription is required.

Hubert Koch
Hubert KochLv2
28 Sep 2019

Unlock all answers

Get 1 free homework help answer.
Already have an account? Log in
Share

Related textbook solutions

Molecular Cell Biology
8 Edition,
Lodish
ISBN: 9781464183393
Biology: Science for Life with Physiology
6 Edition,
Belk
ISBN: 9780134555430
Concepts of Biology
OER Edition,
Openstax
ISBN: 9781938168116
Biology
2nd Edition, 2013
Openstax
ISBN: 9781947172517
Essentials of Biology
5 Edition,
Mader
ISBN: 9781259660269

Related questions

1. You have cloned a human gene along with its promoter and ribosome-binding site in a bacterial cloning vector and then introduced the recombinant plasmid into a bacterial host cell that supports the replication and segregation of the cloning vector. Unfortunately, even though you know the whole human gene sequence is present (by DNA hybridization); you cannot get expression of the gene. Which of the following is NOT a reason why there is no expression?

A. The vector cannot replicate

B. The human promoter is not recognized by the bacterial host cell

C. The ribosome-binding site is not recognized by the bacterial host cell

D. The human gene lacks introns, so it is not transcribed by the host bacterium

2. Restriction endonucleases are:

A. sequence-specific

B. able to recognize any 4-, 6-, or 8-base pair sequence

C. always able to produce single stranded tails

D. only able to cleave bacterial DNA

3. Which of the following is NOT a required characteristic of cloning vectors?

A. Genes for replication of vector DNA and segregation to progeny cells

B. Genes for selection of cells carrying the vector (e.g., antibiotic-resistance)

C. DNA sequence for the introduction of foreign DNA where the integrated DNA does not disable the vector

D. None of the above

4. A poly (T) primer is used to produce cDNA from mammalian mRNA. Which of the following would be used to produce cDNA from bacterial mRNA?

A. A poly (T) primer

B. A random DNA sequence primer

C. A poly (A) primer

D. A poly (GC) primer

By using the techniques of genetic engineering, scientists are able to modify genetic materials so that a particular gene of interest from an animal cell can be incorporated into a bacterial cell for gene expression. Describe a procedure by which this can be done with a cloning vector. Explain the purpose of each step of your procedure. Describe how you could determine whether the bacteria take up the vector and whether the gene was successfully incorporated. Describe an example of how this gene transfer and expression is used in biomedical or commercial applications.

Which are the factors that modify the overall gene differentialexpression by introducing a vector for single-geneoverexpression?

If you overexpress a gene for a protein involved in signaltransduction (e.g., a kinase, scaffold, or receptor) by vector celltransfection, then you overdrive the cell using this signalingpathway, it's useful to isolate the pathway and study them.

Is there any way to modify the overall gene expression or celldifferential expression pattern by gene transfection? I think thiswould work if you delivered a gene for overexpression in proteinsinvolved for RNA processing (e.g., splicing, ribosomal proteins,etc.), RNA transcription (e.g., TFs) or protein translation.

Weekly leaderboard

Home
Homework Help 3,900,000
Biology 460,000
Start filling in the gaps now
Log in