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The presence of aDNA template (e.g., a product from PCR), general transcription factors, and RNA polymerase II allows for the initiation of transcription in vitro. Explain why the initiation of transcription is not possible with only general transcription factors and RNA polymerase II using the genomic DNA as a template.

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Reid Wolff
Reid WolffLv2
28 Sep 2019
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Related questions

1)Which of the following is NOT correct when considering the initiation of transcription in E. coli?

A) The σ subunit of RNA polymerase makes strong contacts with the -10 region of promoters.

B) Only the -10 region of the promoter is required.

C) DNA melting near the transcription start site is essential for promoter recognition.

D) The phosphodiester bonds that are formed are not stable until about 10 nucleotides have been incorporated.

E) The RNA polymerase contacts about 50 bp of DNA.

2)In bacteria, mRNA, tRNA and rRNA are all transcribed by the same RNA polymerase.(TRUE/FALSE)

I am not to sure about this question, could I get some helpplease. Transcription is the RNA polymerase mediated process thatgenerates an RNA molecule (or transcript) using a DNA template.

a) What are the molecular requirements of a transcriptionreaction in a eukaryotic cell? What does each component contributeto the reaction?

b) Compare and contrast the processes of transcription and DNAreplication.

1) In Eukaryotes, repetitive sequence inclues:
A) Centromere sequences
B) Telomere sequences
C) All of the above

2) Satelit DNA:
A) Was orignally discoverd in E. Coli
B) Represents plasmid DNA sequences
C) Contains repetitive DNA sequences
D) Has the same density as main band DNA
E) All of the above

3) Inversions _____ The number of crossover offspring.
A) Decrease
B) Increase
C) Do not change
(I Believe the answer is "C" ^. can you explain why?)

4) In human genome, repetitive DNA is estimated to be ___ of the total genome.
A) .1%
B) 1%
C) 10%
D) 50%
E) 90%

5) In E. Coli, the promoter sequence:
A) Contains a short AT rich sequence
B) Binds transcription factors
C) All of the above

6) In eukaryotic transcription:
A) Transcription factors bind to the promoter and then RNA polymerase binds.
B) Transcription factors bind to RNA polymerase which then binds to the promoter
C) Transcription factors are NOT required to initiate transcription

Explanation for each would help immensely. THANK YOU in advance for your time!!!

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