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28 Sep 2019
Name 2 factors that affect the melting temperature (Tm) of a primer used in PCR. What step in the PCR reaction will be affected most by the Tm of the primers? What will happen if the temperature of this step is too high? too low?
Name 2 factors that affect the melting temperature (Tm) of a primer used in PCR. What step in the PCR reaction will be affected most by the Tm of the primers? What will happen if the temperature of this step is too high? too low?
Irving HeathcoteLv2
28 Sep 2019