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Long-acting insulin was engineered to have a pl that resulted in an insulin protein that was completely soluble at pH 4, but forms insoluble hexamers at a physiological pH of 7.4. What is the pl of a protein?

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Trinidad Tremblay
Trinidad TremblayLv2
28 Sep 2019

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Related questions

a) The isoelectric point (pI) of the hormone insulin is 6.5.Suggest a modification of the insulin Bchain that makes it moreinsoluble at physiological pH, without significantly affecting itsbinding to the Insulin Receptor (IR)

b) Suggest a protein target for a drug thats effects wouldcomplement exogenous delivery of insulin in Type 2 Diabetes.Justify your answer.

c) An insulininduced signaling assay has been developed with theuse of cancer HeLa cells. Assess briefly the reliability of thisassay

Suppose you have cloned a bacterial culture to produce insulin. Why would it be important to use the Bradford assay on a small sample of the insulin that your bacteria produces? Hint: when you produce a water-soluble protein in large quantities, is it easy to know how much protein you have just made?

Suppose you have extracted only one very small sample of protein from a rare plant. Why would it be important to NOT use the Bradford assay on that protein? Hint: Can you reuse the protein after it binds to Coomassie Blue?

QUESTION 20

Which amino acid(s) would you find in the core of a cytosolic protein?

Amino acids that make up the protein's signal sequence

Amino acids with hydrophobic backbones AND sidechains

Amino acids that have been phosphorylated on their side chains

The methionine found at the N-terminus of every protein

Amino acids with non-polar sidechains

QUESTION 21

Why is Ras such an important protein in the cell?

It produces secondary messengers

It opens ligand-gated ion channels

It binds to extracellular ligands

It switches on a cascade of kinases

It phosphorylates the mitogen activated protein kinase kinase kinase

QUESTION 22

Predict the effect of adding a microRNA that binds to the 3' UTR of insulin to a beta cell

The microRNA will interact with the insulin protein via hydrogen bonding

The microRNA will decrease expression of the insulin protein

The microRNA will block transcription of the insulin gene

The microRNA will regulate post-translational modifications of the insulin protein

The microRNA will prevent RNA polymerase from binding to insulin mRNA

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