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B. Fill in the table based on the two images shown: Image 1 Image 2 Gram reaction Shape of cells

1. 2. Fill in the following table describing the steps of the gram stain: Step Reagent Purpose of reagent time Color of gram Positive cells after this step Color of gram Negative cells after this step 1 2 3 4 Answer the following questions:

1. Why is the gram stain technique more valuable than the simple stain technique in the diagnostic laboratory? 2. Suppose distilled water was accidentally used instead of alcohol in the Gram stain procedure. What would be the color of gram positive and gram negative bacteria at the end of the procedure? 3. What is the purpose of the mordant?

4. Why do gram positive bacteria retain the primary stain? 5. Suppose you did a gram stain on a sample of bacteria, and you saw bacilli that were gram positive next to bacilli that looked gram negative. What are two ways you could interpret these results?

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Casey Durgan
Casey DurganLv2
28 Sep 2019

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