What is the complete base composition of a double-stranded eukaryotic DNA that contains 21 % thymidine?

thymidine: 21%

guanosine: _%

adenosine: _%

cytosine: ____%

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Sixta KovacekLv2

28 Sep 2019

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A double-stranded DNA molecule contains 34% guanosine (G). Whatis the complete base composition of the DNA molecule.

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apricotdonkey807

3 H thymidine is incorporated into DNA during synthesis. A culture of rapidly growing asynchronous cells is given 3 H thymidine. The cells are then allowed to grow for 1 hour. At the end of the hour, a sample of the culture is fixed and examined by autoradiography. For the purposes of this experiment, consider that the length of G1 is 15 hours, S is 2 hours, G2 is 3 hours, and M is a half hour. Which result would you predict?

a. All of the cells that are in mitosis, but no others, have incorporated the labeled thymidine.

b. All of the cells, both mitotic and nonmitotic , have incorporated the labeled thymidine.

c . None of the mitotic cells, but some of the non-mitotic cells, have incorporated the labeled thymidine.

d. All of the non mitotic cells have incorporated the labeled thymidine

e. None of the non-mitotic cells have incorporated the labeled thymidine.

DNA Replication

1. Assume that mammalian cells are synchronized in the G1 phase of the cell cycle and then cultured in the presence of tritiated thymidine for several hours until they enter mitosis.

Will both sister chromatids contain the radioactive label?

2.Will the label be contained in one strand or both strands of DNA in a sister chromatid?

3. If the cells are then allowed to complete two additional cell division cycles in the absence of tritiated thymidine, what will be the distribution of radioactive thymidine within chromosomes at the metaphase stage during each cell division cycle?