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1.Can methylation of nucleotides play a role in DNA replication? _____Yes or No

2. If so, what role does it play? (PICK ONE OF THE 4 CHOICES)

Removal of methyl groups from nucleotides is concurrent with the conversion of dNTPs to dNMPs during DNA synthesis.

Lack of methylation on the synthesized DNA strand can be recognized by repair enzymes, thus permitting selective base pair mismatch repair.

Lack of methylation on the parental DNA strand can be recognized by repair enzymes, thus permitting selective base pair mismatch repair on the newly synthesized strand.

Methylation does not play a role in replication.

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Nelly Stracke
Nelly StrackeLv2
28 Sep 2019
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Related questions

1.When a protein binds to DNA at a site defined by a particular nucleotide sequence, with which parts of the DNA does the protein primarily interact?

A. the phosphate and deoxyribose groups in the DNA backbone

B. the phosphate and deoxyribose groups in the DNA backbone, and hydrophobic interactions with the nucleotide bases

C. nucleotide bases within the major grooves of the DNA

D. nucleotide bases within the major and minor grooves of the DNA

E. none of the above

2. Which of the following proteins binds to DNA with sequence specificity?

A. POI Ill holoenzyme

B. DnaA

C. DnaB

D. Dam methylase

E. Both B and D

3. Which of the following is NOT a functional parallel among all known cellular systems of DNA replication?

A. Replication is initiated at specific sites

B. Replication is continuous on the leading strand and discontinuous on the lagging strand

C. DNA replication is semiconservative

D. Replication forks move away from the origin in one direction

E. Nucleases, polymerases and ligases replace RNA primers with DNA and seal the remaining nick

4. A DNA polymerase cannot synthesize a DNA strand de novo (from the beginning) from a mixture of dNTPs. Which of the following explains the role of the primer in the reaction promoted by this enzyme?

A. The primer is an initiation protein with attached nucleotides that binds to the template and to which new nucleotides are added to the 3 ' end.

B. The primer is an initiation protein with attached nucleotides that binds to the template and to which new nucleotides are added to the 5' end.

C. The primer is a preexisting strand of RNA or DNA to which new nucleotides are added to the 3' end.

D. The primer is a preexisting strand of RNA or DNA to which new nucleotides are added to the 5' end.

E. The primer is a preexisting strand of DNA synthesized by DNA pol I, prior to replicative DNA synthesis by DNA pol Ill.

5. Universal features of DNA polymerases include:

A. 5'-3' exonuclease function.

B. Formation of an 'closed' conformation of the polymerase when the template-nucleotide base pair is correct.

C. Use of a single magnesium ion to orient reacting molecules and assist in dissipation of the negative charge that develops during nucleotidyl transfer.

D. Alignment of conserved residues in the active site for attack of the DNA primer by the 3 'OH on the incoming nucleotide.

E. Continuous unbinding and rebinding of the polymerase to the DNA template as each nucleotide is added.

If one is testing a goodness of fit to a 9:3:3:1 ratio, how many degrees of freedom would be associated with the Chi-square?

1

2

3

4

not enough information to determine

Which of the following choices does not describe a type of posttranslational modification of proteins?

The amino terminal methionine may be removed.
Glycoproteins are produced by the attachment of phosphates to newly synthesized proteins.
Precursor proteins are cleaved and trimmed by protease enzymes to produce a functional protein.
Molecular chaperones help proteins fold correctly.
Amino acids in the amino terminal of the protein can be acetylated.

A eukaryotic DNA sequence that affects transcription at distant promoters is called a(n)

long distance promotor
regulator
operon
enhancer

DNA methylation may be a significant mode of genetic regulation in eukaryotes. Meythylation refers to

altering RNA polymerase activity by adding methyl-groups
changes in DNA-DNA hydrogen bonding
altering translation activity via methylated tRNAs
adding methyl groups to certain cytosines in DNA

If a nucleotide lacks a 3’-OH group, what will happen?

it’s transformed into RNA
another nucleotide can bond at the 3’ site during replication
replication will halt
no effect

Which of the following describe posttranscriptional modifications commonly seen in mRNA of eukaryotes?

5’ capping, 3’-poly-A tail addition, splicing
3’ capping, 5’ poly-A tail addition, splicing
heteroduplex formation
5’ capping, 3’polyA tail addition, removal of exons

In birds, females are the heterogametic sex. This means that

females have chromosomes in pairs.
emales produce eggs.
females determine the gender of their offspring.

males have two identical sex chromosomes (ZZ)

An anticodon on a tRNA has the sequence 5'–GCA–3'. What would be the effect on translation if the G in the anticodon were mutated to a U?

The anticodon could pair to the serine codon 5'–UCA–3', and a cysteine would be incorporated at all points where a serine should have been incorporated.
The anticodon could pair to the threonine codon 5'–ACU–3', and a threonine would be incorporated at all points where a cysteine should have been incorporated.
The anticodon could pair to the serine codon 5'–AGU–3', and a serine would be incorporated at all points where a cysteine should have been incorporated.
The anticodon could pair to the stop codon 5'–UGA–3', and instead of stopping translation, a cysteine would be incorporated and translation could continue.

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