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28 Sep 2019
In order to isolate the protein, seeds were ground and extracted with water. The proteins in the extract were precipitated with hydrochloric acid and the pellet isolated by centrifugation was discarded. The supernatant was heated to 70°C to remove heat-labile proteins, then lyophilized (freeze-dried). The lyophilized powder was dissolved in a small amount of ammonium acetate buffer at pH = 5 and the sample was loaded onto a Sephadex G-251 gel filtration column. The elution profile showed four peaks. Most of the BN protein eluted in the first peak. a. On what basis is separation achieved on the Sephadex gel filtration column? b. Compare the BN protein to other proteins found in the B. nigra seeds.
In order to isolate the protein, seeds were ground and extracted with water. The proteins in the extract were precipitated with hydrochloric acid and the pellet isolated by centrifugation was discarded. The supernatant was heated to 70°C to remove heat-labile proteins, then lyophilized (freeze-dried). The lyophilized powder was dissolved in a small amount of ammonium acetate buffer at pH = 5 and the sample was loaded onto a Sephadex G-251 gel filtration column. The elution profile showed four peaks. Most of the BN protein eluted in the first peak. a. On what basis is separation achieved on the Sephadex gel filtration column? b. Compare the BN protein to other proteins found in the B. nigra seeds.
Casey DurganLv2
28 Sep 2019
