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How would you differentiate between Edwardsiella tarda and Klebsiella pneumoniae? Describe the expected growth outcome and appearance on the media that you choose

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Tod Thiel
Tod ThielLv2
28 Sep 2019

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Related questions

How would you us the media to help identify S. Marcescens and E. coli if they were growing together in a tube of nutrient broth? Which technique would you use to help you distinguished between colonies of the two bacteria? If you were trying to obtain pure cultures of these bacteria from the tube of nutrient broth what selective and differential media might you use and how would you separate and purify the two cultures of bacteria?

E. aerogenes on the selective and differential media, describe how you might use this information to predict the Gram reaction for this bacterium?

How would you differentiate between Edwardsiella tarda and Klebsiella pneumoniae? Describe the expected growth outcome and appearance on the media that you choose.

For each of the scenarios below use this list of possibilities to choose the most likely unknown organism.

Consider the following possibilities: E. coli, K rhizophila, S. marcescenes, S. aureus, B. subtilis,

S. epidermidis, S. pyogenes, M. luteus, E. aerogenes, P. aeruginosa, P. vulgaris.

Scenario-1

Even though we did not use Mannitol Salt media in our experiment, knowing what you do about its properties predict the unknown for this scenario. Data: microscopic observation at 1000X = cocci in cluster arrangement, Growth was observed, on Nutrient agar, PEA media and Mannitol Salt media while no growth was seen on EMB media. Colonies on Mannitol Salt appeared white.

Organism = _________________________________________

Scenario-2

Data: microscopic observation at 1000X = cocci in cluster arrangement, Growth was observed on Nutrient agar, PEA media and Mannitol Salt media while no growth was seen on EMB media. Colonies on Mannitol Salt appeared yellow.

Organism = __________________________________________

Scenario-3

Data: microscopic observation at 1000X = small individual rods, growth on nutrient Agar, EMB media, but no growth on PEA media. Growth appearance on EMB was that of metallic green colonies.

Organism = __________________________________________

Scenario-4

Data: microscopic observation at 1000X = individual rods, growth on Nutrient Agar, EMB media, but no growth on PEA media. Growth appearance on EMB was that of thick, mucoid, pink colonies.

Organism = ___________________________________________

Scenario-5

Data: microscopic observation at 1000X = rods in chains, diplo bacilli and individual rods, growth on Nutrient Agar, and PEA media, no growth was observed on Mannitol Salt or EMB media.

Organism = ____________________________________________

1)Could you identify your unknown from the Gram Stain alone? _____ How did you identify it?

8. The supervisor wants you to predict what growth will occur and on what plates, based on what you see microscopically in the gram stain from a fecal sample.

http://homepage.usask (9.19.09)

a. What organism do you think it is?

b. What media will it grow on and how do you incubate it?

9. One of the enterotoxins produced by enterotoxigenic Eschericha coli in traveler’s diarrhea is similar to a toxin produced by __________________________.

a. How does this toxin work?

10. You have narrowed the id of an organism from a fecal sample down to either Salmonella or Citrobacter.

a. What media would you use to differentiate the two organisms

b. Describe the growth on the media for each organism.

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