1. You smear a small a mount of culture onto a strip containing a reagebt that tests for the presence of oxidase. The inoculated spot turns dark blue within 20 seconds. What does i mean?
a. The culture deos not contain oxidase
b. The culture is likely an aerobe
c. The culture is likely an anaerobe
d. The culture does not contain catalase
E. None of the above
2. True or False
a. The catalase enzyme is found in all obligate aerobes and facultatuve anaerobes to deal eith H2O2
b. An exergonic reaction requires energy input and must be coupledwith ATP hydrolysis in order to proceed
c. The TCA cycle must "turn twice" in order to complete the oxidation of one glucose molecule.
d. Allosteric regulation occurs when an enzyme has a site that can bind an effector molecule seperate from the active site.
e. Some bacteria use desulfurases to produce hydrogen sulfide from he breakdown of some amino acids.
f. A 1/10 dilution can be made by adding 1 mL sample to 10mL diluent
3. You obtain the following results after incubation of a bacterial starin in tge indicated carbohydrate fermemtation sugar media:
Sugar: Color of Medium Durham tube results
Glucose Yellow No bubble
Maltose Red Bubble
Mannitol Yellow No bubble
Lactose Yellow Bubble
Sucrose Red No Bubble
Which sugars cannot be fermented by this bacterial strain?
a. glucose and mannitol
b. maltose and lactose
c. lactose
d. maltose and sucrose
e. none of the choices are correct
1. You smear a small a mount of culture onto a strip containing a reagebt that tests for the presence of oxidase. The inoculated spot turns dark blue within 20 seconds. What does i mean?
a. The culture deos not contain oxidase
b. The culture is likely an aerobe
c. The culture is likely an anaerobe
d. The culture does not contain catalase
E. None of the above
2. True or False
a. The catalase enzyme is found in all obligate aerobes and facultatuve anaerobes to deal eith H2O2
b. An exergonic reaction requires energy input and must be coupledwith ATP hydrolysis in order to proceed
c. The TCA cycle must "turn twice" in order to complete the oxidation of one glucose molecule.
d. Allosteric regulation occurs when an enzyme has a site that can bind an effector molecule seperate from the active site.
e. Some bacteria use desulfurases to produce hydrogen sulfide from he breakdown of some amino acids.
f. A 1/10 dilution can be made by adding 1 mL sample to 10mL diluent
3. You obtain the following results after incubation of a bacterial starin in tge indicated carbohydrate fermemtation sugar media:
Sugar: Color of Medium Durham tube results
Glucose Yellow No bubble
Maltose Red Bubble
Mannitol Yellow No bubble
Lactose Yellow Bubble
Sucrose Red No Bubble
Which sugars cannot be fermented by this bacterial strain?
a. glucose and mannitol
b. maltose and lactose
c. lactose
d. maltose and sucrose
e. none of the choices are correct
