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1.) what are the applications of restriction endonucleases ?

2.) what are the essential components of a plasmid cloning vector and a gene expression vector ?

Answer questions 1&2 please !

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Jamar Ferry
Jamar FerryLv2
28 Sep 2019

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Related questions

1. What are essential elements for a useful plasmid vector? Why is it advantageous to have a restriction site for an endonuclease in a plasmid vector? Can a piece of foreign DNA be inserted into a plasmid if they have been cut with different restriction endonucleases?

2. Briefly explain the molecular basis of cDNA synthesis. Why is cDNA synthesis considered to be such an important technique in molecular biology?

Gene cloning is a bit of a different process. Describe itbriefly in a paragraph. Be sure to use the key words: rDNA, vector,plasmid, restriction enzyme, DNA ligase.

1. You have cloned a human gene along with its promoter and ribosome-binding site in a bacterial cloning vector and then introduced the recombinant plasmid into a bacterial host cell that supports the replication and segregation of the cloning vector. Unfortunately, even though you know the whole human gene sequence is present (by DNA hybridization); you cannot get expression of the gene. Which of the following is NOT a reason why there is no expression?

A. The vector cannot replicate

B. The human promoter is not recognized by the bacterial host cell

C. The ribosome-binding site is not recognized by the bacterial host cell

D. The human gene lacks introns, so it is not transcribed by the host bacterium

2. Restriction endonucleases are:

A. sequence-specific

B. able to recognize any 4-, 6-, or 8-base pair sequence

C. always able to produce single stranded tails

D. only able to cleave bacterial DNA

3. Which of the following is NOT a required characteristic of cloning vectors?

A. Genes for replication of vector DNA and segregation to progeny cells

B. Genes for selection of cells carrying the vector (e.g., antibiotic-resistance)

C. DNA sequence for the introduction of foreign DNA where the integrated DNA does not disable the vector

D. None of the above

4. A poly (T) primer is used to produce cDNA from mammalian mRNA. Which of the following would be used to produce cDNA from bacterial mRNA?

A. A poly (T) primer

B. A random DNA sequence primer

C. A poly (A) primer

D. A poly (GC) primer

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