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periwinklekoala410Lv1

28 Sep 2019

Explain the polymerase chain reaction process. Use terms primer,template, DNA, nucleotides, and DNA polymerase.

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shiLv10

22 May 2022

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Hubert KochLv2

28 Sep 2019

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Related questions

1) Recombinant DNA technology can be used for

Question 1 options:

1)	correcting genetic defects.

2)	the industrial production of useful proteins.

3)	producing transgenic organisms.

4)	all of the above

5)	constructing mutant proteins.

4) DNA sequencing using the Sanger method requires

Question 4 options:

1)	template, primer, DNA polymerase, mRNA, dNTPs, ddNTPs.

2)	template, primer, DNA polymerase, mRNA, dNTPs.

3)	template, primer, DNA polymerase, rRNA, dNTPs, ddNTPs.

4)	template, primer, DNA polymerase, dNTPs, ddNTPs.

5) none of the above

5) Mutations leading to changes that can be inherited by the next generation have to be introduced into at the _______ level.

Question 5 options:

protein

tRNA

rRNA

DNA

mRNA

greenmole122

Place the following steps of DNA replication in right chronological order.
1. Primase adds an ribonucleotide primer
2. Initiator proteins bind the origin of replication and helicase breaks hydrogen bonds between complementary bases of double stranded template DNA
3. DNA polymerase III extends by complementary base pairing deoxyribonucleotides to the template strands
4. Ligase phosphodiester bonds the nucleotides to connect okazaki fragments nad connect fragments at the origin and where the "crotches or forks" collide
5. DNA polymerase I removes RNA primer and replaces with Deoxyribonucleotides

plumsardine151

1) In general, the length of the primer is 18 -25 nucleotides. Why? 2) Thermostable DNA polymerase commonly used in PCR is Taq polymerase and Pfu polymerase. Compare these characteristics.

please explain in details!!! thank you