- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.
Step 1.

- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.
Step 2.

- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.
Step 3.

- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.
Step 4.

- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.
Step 5.

- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.
Step 6.

- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.
Step 7.

- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.
Step 8.

- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.
Step 9.

- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.
Step 10.

- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.
Step 11.

- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.
Step 12.

- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.
Step 13.

- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.
Step 14.

- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.
Step 15.

- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.
Step 16.

A.
Don appropriate personal protective equipment (PPE).

B.
Aseptically transfer 1 mL from dilution tube 2 to dilution tube 3 and mix well. This is a 10^-5 cumulative dilution.

C.
Aseptically transfer 1.0 mL from dilution tube 4 to dilution tube 5 and mix well. This is a 10^-7 cumulative dilution.

D.
Aseptically transfer 0.1 mL from dilution tube 1 to dilution tube 2 and mix well. The cumulative dilution of the culture is 10^-4.

E.
Allow the inocula to soak into the plates before proceeding.

F.
Aseptically transfer 0.1 mL from dilution tube 2 to plate A₁. Using the spread plate technique, disperse the sample evenly over the entire surface of the agar.

G.
Aseptically transfer 1 mL from dilution tube 3 to dilution tube 4 and mix well. This is a 10^-6 cumulative dilution.

H.
Obtain eight plates, organize them int four pairs and label them A₁, A₂, B₁, B₂, etc.

I.
Keep accurate records in your lab notebook.

Clean up the lab benches, doff PPE, and wash hands.

J.
Repeate the procedure with plate A₂ and label both plates "10^-5mL of original sample," or simply "10^-5"

Notice that transferring 0.1 mL counts as a dilution!! The tube was a 10^-4 dilution, but the plate is a 10^-5 dilution!!

K.
Invert the plates so they are "agar up" to prevent condensation from ruining the plates.

L.
Repeat the aseptic transfer, spread plate technique, and label schematic for dilutions tubes 3, 4, and 5, and plates B, C, and D.

M.
Incubate the plates at 35â for 24 to 48 hours.

N.
Obtain five dilution tubes and label them 1â5, respectively. Make sure they remain covered until needed.

O.
Aseptically add 9.9 mL sterile water to dilution tubes 1 & 2. Cover when finished. Aseptically add 9.0 mL sterile water to dilution tubes 3, 4, and 5. Cover when finished.

P.
Mix the broth culture an aseptically transfer 0.1 mL to dilution tube 1. Mix dilution tube 1 well. This is a 10^-2 dilution

For the following strand of DNA, use the table below to provide: bases for the complementary mRNA and the associated amino acids. What do you notice about the genetic code for proline in the peptide? How would the loss of the first G or switching that G to a T affect the structure and function of this protein?

DNA: GGC CTA CAA GCC GGA ACA GTT ACC GCG TAA CGA

RNA: ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ???

Amino Acid:??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ???
(3 Letters)

Genetic Code Table (mRNA)

1st position
(5' end)
2nd position (middle)
3rd position
(3' end)

U C A G

U Phe F
Phe F
Leu L
Leu L Ser S
Ser S
Ser S
Ser S Tyr Y
Tyr Y
STOP
STOP Cys C
Cys C
STOP
Trp W U
C
A
G

C Leu L
Leu L
Leu L
Leu L Pro P
Pro P
Pro P
Pro P His H
His H
Gln Q
Gln Q Arg R
Arg R
Arg R
Arg R U
C
A
G

A Ile I
Ile I
Ile I
Met M Thr T
Thr T
Thr T
Thr T Asn N
Asn N
Lys K
Lys K Ser S
Ser S
Arg R
Arg R U
C
A
G

G Val V
Val V
Val V
Val V Ala A
Ala A
Ala A
Ala A Asp D
Asp D
Glu E
Glu E Gly G
Gly G
Gly G
Gly G U
C
A
G

turquoisewater-buffalo47

Match the Words chose one letter for each number

1-Plasmodium

2-Stable resting stage of some Gram-positive bacteria

3-Alphaproteobacteria

4-Firmicutes

5-Entamoeba

6-Capsid

7-Bacillus

8-Bacteriophage

9-Hyphae

10-Retroviruses

A.
Acid fast

B.
Prions

C.
Fungi

D.
HIV , +ssrna

E.
Spherical

F.
Lytic Replication

G.
Coccobacillus

H.
Brucella

I.
Protozoa

J.
Viroid

K.
Escherichia coli

L.
Rod-shaped

M.
Eukaryote

N.
Vary in shape and size

O.
Some gram-positive species

P.
Endospore

Q.
Diphtheria

R.
Clostridia

S.
Virus

T.
Lobe-shaped pseudopodia

Question

- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 1.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 2.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 3.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 4.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 5.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 6.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 7.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 8.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 9.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 10.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 11.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 12.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 13.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 14.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 15.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 16.

A.	Don appropriate personal protective equipment (PPE).
B.	Aseptically transfer 1 mL from dilution tube 2 to dilution tube 3 and mix well. This is a 10^-5 cumulative dilution.
C.	Aseptically transfer 1.0 mL from dilution tube 4 to dilution tube 5 and mix well. This is a 10^-7 cumulative dilution.
D.	Aseptically transfer 0.1 mL from dilution tube 1 to dilution tube 2 and mix well. The cumulative dilution of the culture is 10^-4.
E.	Allow the inocula to soak into the plates before proceeding.
F.	Aseptically transfer 0.1 mL from dilution tube 2 to plate A₁. Using the spread plate technique, disperse the sample evenly over the entire surface of the agar.
G.	Aseptically transfer 1 mL from dilution tube 3 to dilution tube 4 and mix well. This is a 10^-6 cumulative dilution.
H.	Obtain eight plates, organize them int four pairs and label them A₁, A₂, B₁, B₂, etc.
I.	Keep accurate records in your lab notebook. Clean up the lab benches, doff PPE, and wash hands.
J.	Repeate the procedure with plate A₂ and label both plates "10^-5mL of original sample," or simply "10^-5" Notice that transferring 0.1 mL counts as a dilution!! The tube was a 10^-4 dilution, but the plate is a 10^-5 dilution!!
K.	Invert the plates so they are "agar up" to prevent condensation from ruining the plates.
L.	Repeat the aseptic transfer, spread plate technique, and label schematic for dilutions tubes 3, 4, and 5, and plates B, C, and D.
M.	Incubate the plates at 35â for 24 to 48 hours.
N.	Obtain five dilution tubes and label them 1â5, respectively. Make sure they remain covered until needed.
O.	Aseptically add 9.9 mL sterile water to dilution tubes 1 & 2. Cover when finished. Aseptically add 9.0 mL sterile water to dilution tubes 3, 4, and 5. Cover when finished.
P.	Mix the broth culture an aseptically transfer 0.1 mL to dilution tube 1. Mix dilution tube 1 well. This is a 10^-2 dilution

For the following strand of DNA, use the table below to provide: bases for the complementary mRNA and the associated amino acids. What do you notice about the genetic code for proline in the peptide? How would the loss of the first G or switching that G to a T affect the structure and function of this protein?

DNA: GGC CTA CAA GCC GGA ACA GTT ACC GCG TAA CGA

RNA: ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ???

Amino Acid:??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ???
(3 Letters)

Genetic Code Table (mRNA)

1st position (5' end)	2nd position (middle)				3rd position (3' end)
1st position (5' end)	U	C	A	G	3rd position (3' end)
U	Phe F Phe F Leu L Leu L	Ser S Ser S Ser S Ser S	Tyr Y Tyr Y STOP STOP	Cys C Cys C STOP Trp W	U C A G
C	Leu L Leu L Leu L Leu L	Pro P Pro P Pro P Pro P	His H His H Gln Q Gln Q	Arg R Arg R Arg R Arg R	U C A G
A	Ile I Ile I Ile I Met M	Thr T Thr T Thr T Thr T	Asn N Asn N Lys K Lys K	Ser S Ser S Arg R Arg R	U C A G
G	Val V Val V Val V Val V	Ala A Ala A Ala A Ala A	Asp D Asp D Glu E Glu E	Gly G Gly G Gly G Gly G	U C A G

turquoisewater-buffalo47

Match the Words chose one letter for each number 1-Plasmodium
	2-Stable resting stage of some Gram-positive bacteria
	3-Alphaproteobacteria
	4-Firmicutes
	5-Entamoeba
	6-Capsid
	7-Bacillus
	8-Bacteriophage
	9-Hyphae
	10-Retroviruses

A.	Acid fast
B.	Prions
C.	Fungi
D.	HIV , +ssrna
E.	Spherical
F.	Lytic Replication
G.	Coccobacillus
H.	Brucella
I.	Protozoa
J.	Viroid
K.	Escherichia coli
L.	Rod-shaped
M.	Eukaryote
N.	Vary in shape and size
O.	Some gram-positive species
P.	Endospore
Q.	Diphtheria
R.	Clostridia
S.	Virus
T.	Lobe-shaped pseudopodia

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