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1. why does the dye wash out of the vegetative cells duringdecolonization ? ( Schrieffer-fulton endospore stain )

2. even though endospore may be observed as a result of routineSimple or Gram Staining , it is still necessary to perform theSchaeffer-Fulton stain, Why ?

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Hubert Koch
Hubert KochLv2
28 Sep 2019

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Related questions

These Questions Are in regards to Stainingtechniques.

Endospores

1) Under what conditions are endospores usually formed?

2) How do Bacillus and Clostridium genera differ in their oxygenpreference?

3)After Staining, why do endospores appear green but the rest ofthe cell pink?

4) Name 3 diseases caused by anaerobicendospore forming organisms and one caused byaerobic endospore forming organisms.

Acid Fast Stain

1) The mycobacteria appear fuchsia with an Acid Fast Stain andpurple with a Gram Stain. Explain his phenomenon.

2) Why is it desireable to combine S. aureus with acid fastorganisms such as M. smegmatis when applying the Acid Fast stainingtechnique?

3) Name two human diseases and their causative agents where theAcid Fast stain is used in diagnosis.

Would greatly appreciate as detailed of an answer aspossible.

Microbiology

1) For gram positive cell walls, does the cell wall itself carry a negative charge and regulate movement of cations or is those characteristics of teichoic acid?

2) For basic dyes, it's a cation that stains a negatively charged microorganism?

3) Is the sole purpose for gram staining to differentiate between species?

4) Is carbolfuchsin a basic or acidic dye?

5) in special staining, do all tests (capsule, flagella, and endospore) show the halo effect?

6) in acid-fast staining, is there any interaction of charges?

7)the interaction between crystal violet and gram (-) bacteria. I assumed since gram (-) bacteria don't hold the dye, they do not interact. Can you explain how they do?

1. At what point in the Gram Stain procedure would you be able to differentiate Gram-Positive and Gram-Negative cells? (Eg. after the crystal violet is applied, after the Safranin is applied?) Explain why.

2. Could you switch out the Safranin dye with another? Think carefully and explain.

3. Gram-Variable organisms will not produce a consistent gram result. Give two different reasons an organism would be Gram-Variable, give an example of an organism (Genus and species name) and an alternate staining method that could be used to identify that organism for each reason.

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