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28 Sep 2019
A conjugation experiment is performed to map the location of three genes involved in the ability to synthesize three amino acids, histidine (His), tryptophan (Trp), and leucine (Leu). An Hfr strain with genotype met-his+leu+trp+ is mated with a met+his-leu-trp- recipient. The met marker, which (confers the ability to synthesize the amino acid methionine) is known to be transferred very late. After a short mating the cells are broken apart and plated on four different growth media. The amino acids present in the growth medium and the number of colonies observed on each are the following:
His, Trp: 250
His, Leu: 50
Leu, Trp: 500
His only: 10
A) What is the purpose of the met- mutation in thisexperiment?
Please provide explanations.
A conjugation experiment is performed to map the location of three genes involved in the ability to synthesize three amino acids, histidine (His), tryptophan (Trp), and leucine (Leu). An Hfr strain with genotype met-his+leu+trp+ is mated with a met+his-leu-trp- recipient. The met marker, which (confers the ability to synthesize the amino acid methionine) is known to be transferred very late. After a short mating the cells are broken apart and plated on four different growth media. The amino acids present in the growth medium and the number of colonies observed on each are the following:
His, Trp: 250
His, Leu: 50
Leu, Trp: 500
His only: 10
A) What is the purpose of the met- mutation in thisexperiment?
Please provide explanations.
Nelly StrackeLv2
28 Sep 2019