Compare and contrast protein coding genes ineukaryotic AND prokaryotic systems.

**-Consider thepromoters

-Consider the interaction of the transcriptionalmachinery with the promoters during intitiation and termination oftranscription.

IF YOU DO NOT KNOW DO NOT** ANSWER! Rememberparagraph form and please answer the questionCOMPLETELY. Make a list if you must. :)

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Hubert KochLv2

29 Sep 2019

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In this assignment, you will be concerned with de novo protein-coding gene annotation (identifying the location and extent of all protein-coding genes) of the M. genitalium prokaryotic genome using only the genome as input. In short, your goal is to identify which ORFs are CDSs. The best bioinformatics annotation pipelines use all kinds of additional evidence, including knowledge of genes in other genomes, to achieve the best annotations, but de novo annotation is usually a part of or at least has informed modern annotation pipelines

Plan and write pseudocode for an algorithm to solve the problem. Answer the following ques-tions in the process. How do you deï¬ne an ORF? How to you ï¬nd all ORFs in the M. genitalium genome? What signal will you use to identify CDS? How will you compute this signal? How will you use the signal to make decisions about which ORFs are CDSs?

Answer the following question regarding protein synthesis indetailed paragraph form:

With regard to the initiation of protein translation,describe in detail how the appropriatestart codons are identified inboth prokaryotes and eukaryotes.Be sure to identify the factors involvedin recognition of elements found in thetranscripts.

IF YOU DO NOT KNOW DO NOT ANSWER!!! Rememberdetailed paragraph form please!!! Answercompletely!!!

viridiangoat126

My instructor sent me this in an email to study for a final.Please help me construct the best possible answer for this as Ihave not written a satisfactory answer to the first question allsemester.

1) As you know the final does include a comprehensive componentregarding the gene and genome. (80 pts)

This first question has to be extremely detailed. For example,"A gene is a unit of inheritance. The gene carries information forit to be passed from one generation to the next. When you combine agene with the environment you are five the necessities for aphenotype. There are many gene types and elements such as the genesfor RNAs that carry information to make proteins, genes fortransfer RNAs that carry amino acids to the ribosomes, genes forribosomal RNAs that are required for structure, function andactivities of a ribosome, genes for small nuclear RNAs that make upthe spliceosome and help to splice introns, and the genes fortelomerase RNA component (TERC). A gene must also include manyparts such as introns, exons, core promoters, regulatory promoters,activators, downstream promoter enzymes (DPE), and many others.These parts of the genes as well as the different gene types mustallow for some variation in certain conditions to allow evolutionto occur. A gene must also be able to be passed on, or inheritedfrom one generation to the next." Figures and drawings are alsoappreciated by my instructor. If you could basically just throweverything you can think of within a gene or a genome at thisquestion that would be greatly appreciated.

2) You will also need to study genetic mapping, DNA as a digitalstorage device, X chromosome inactivation and synthetic biology.(35 pts)

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