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In the Anfinsen protein folding experiment the sequence of removing urea or BME makes a difference for functional refolding of the protein. Explain why removing BME first leads to a nonfunctional enzyme, but removing urea first leads to a functional enzyme. Why does adding small amounts of BME alone to the solution with the nonfunctional enzyme "rescue" the function?

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Beverley Smith
Beverley SmithLv2
29 Sep 2019

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INSTRUCTIONS

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