Log in
Biology
1
answer
0
watching
56
views

when you add a photograph of a plant to your lab notebook, what must you to to that added material to ensure that your notebook is correct and legal.

Watch
1
answer
0
watching
56
views

For unlimited access to Homework Help, a Homework+ subscription is required.

Bunny Greenfelder
Bunny GreenfelderLv2
29 Sep 2019

Unlock all answers

Get 1 free homework help answer.
Already have an account? Log in
Share

Related textbook solutions

Molecular Cell Biology
8 Edition,
Lodish
ISBN: 9781464183393
Biology: Science for Life with Physiology
6 Edition,
Belk
ISBN: 9780134555430
Biology
2nd Edition, 2013
Openstax
ISBN: 9781947172517
Concepts of Biology
OER Edition,
Openstax
ISBN: 9781938168116
Essentials of Biology
5 Edition,
Mader
ISBN: 9781259660269

Related questions

In defining the pink synthesis pathway, you find an intriguing large protein, Alecia. You scribble down the mRNA sequence in your lab notebook with a flair pen. Your lab partner bumps into you, and you accidently spill a bottle of liquid yeast media onto your notebook, blurring all but the middle of the mRNA sequence: 5’-AUGUGUAGGCACUGA-3’. What is the amino acid sequence of this region of the protein? Explain your answer.

Graded Assignment
Lap Report: Topographic Maps
Answer the questions. When you are finished, submit this assignment to your teacher by the due date for full credit.
Total score: of 35 points
Map and Profile
(Score for Question 1: of 14 points)
1. Draw your topographic map in the space below. Include the name of the island, scale, legend, compass direction, vertical units and contour interval on your map. If you need more room, you can use a full sheet of paper.
Answer:
(Score for Question 2: of 12 points)
2. Make your profile using your topographic map and this profile scale. If you need more room, you can use a sheet of lined notebook paper.

For Discussion (2-3 paragraphs) • What was the concentration of your pre-diluted NPs? • Determine the effect of NPs on brine shrimp lethality. • Determine the effect of NPs on cell viability. • What is (are) the control(s) in this experiment? What is its purpose? • Based on your data in this lab, what is a safe concentration for brine shrimp Threshold Level of Toxicity (TTL)? • Often indicator species are used to study the overall health of an ecosystem. If you were to study an ecosystem containing brine shrimp, would you use it as an indicator species? Why or Why not? Explain your reasoning. • What possible sources of error were present in this experiment?

Introduction

LD50 is a commonly used term to describe acute toxicity: LD stands for Lethal Dose and 50 is used because the dose could kill 50% of the animals exposed to the chemical. For LD50 tests, the animals are given specific amounts of the chemical, orally, by injection or dermally. The value is then reported along with the method of administration and the type of animal used in the test. LD50 is typically reported in milligrams or grams per kilogram of body weight of the animal. The lower the LD50 is, the higher the toxicity of the chemical would be. This information is always provided in Safety Data Sheet (SDS) of chemicals in the “toxicity data” category. A similar measure, the LC50 is usually used to describe the ecological hazards of chemicals. LC stands for lethal concentration that could kill 50% of the animals after 24-96 hours. This value is always provided in Safety Data Sheet (SDS) of chemicals in the “Ecological Data” category. In this lab, you will be measuring LC50 or LD50 of iron oxide nanoparticles using brine shrimp. Iron oxide nanoparticles are magnetic. Application of magnetic nanoparticles ranges from magnetic storage media to medical industry where they can be used for targeted drug delivery or as contrast agents in MRI. Thus, measuring environmental and biological toxicity of these nanoparticles is crucial. To test toxicity of any such reagents on small organisms, Daphnia and brine shrimps (also known as Artemia) can be used. Artimia are found in salt lakes around the world. They are important live food for aquarium fish. In this lab, you will use brine shrimp to measure toxicity of the nanoparticles. Trypan blue is a stain used to assess cell viability. It is able to diffuse through cell membranes of dead cells but is unable to permeate live cells. This stain can be used to assess cell survival 1 Fall 2017 following exposure to chemicals, nanoparticles and experimental drugs. The ratio of live: dead cells can be used as a preliminary assessment of cytotoxicity and translated to tissue viability.

Methods Part A: Serial Dilution of NP Solution 1) Label 5 test tubes as 0, 1:1, 1:10, 1:100, 1:1000 2) Add 2mL of seawater to tube 0 3) Add 2mL of specific group stock solution to tube 1:1 and add 2mL of seawater to it. 4) Transfer 1mL of stock solution to tube 1:10 and add 9mL of seawater to this tube 5) Transfer 1mL of 1:10 solution to the tube labeled as 1:100 and add 9mL of seawater to 
this tube 6) Transfer 1mL of 1:100 solution to the test tube labeled as 1:1000 and add 9mL of 
seawater to this tube Part B: Preparation of test environments 1) Label 5 wells of 6-well plate as control (0), 1:1, 1:10, 1:100 and 1:1000 2) Label 3 petri dishes as: control (0), 1:1 and 1:1000 
NOTE: Be sure to include your initials on all plates 3) Transfer 2mL of each solution from the corresponding test tube to the respective wells on your six-well plate and each petri dish Part C: Preparing specimens for the Trypan blue cell viability test 1) Transfer 2 brine shrimp into each of the pre-labeled petri dishes containing the solution of NPs and seawater (0, 1:1 & 1:1000) and set a timer for 30 minutes. 2) Transfer a single brine shrimp into the unlabeled petri dish with a drop (~50ul) of seawater and use the dissecting microscope to make observations related to the behavior and morphology of untreated brine shrimp.
Make observations in lab notebook of all samples Part D: Preparation of specimens for the toxicity assay 1) 2) 3) 4) 5) Add 10 brine shrimp into each well in the 6 -well plate Make observations in lab notebook Using a dissecting microscope (if needed), count the number of brine shrimp in each well (alive shrimps).
Record your data in your notebook using the graphic below as a template. Close the plate and store it for next observation.
Observations must be done within 24h-96h
Note: There will be times allocated and a sign up paper for observations Part E: Trypan blue viability test 2 Fall 2017 1) Label 3 glass slides to match the petri dishes prepared in Part C. 2) Transfer 1 brine shrimp from each petri dish onto the corresponding slide. 
Make observations in your lab notebook 3) Add a drop (~60 μl) of PBS on top of each specimen 4) Add 60 μl of Trypan blue to your shrimp and incubate for 1-2 minutes at room 
temperature. 5) Wick away excess liquid 6) Add 100 μl PBS to sample and observe (while alive!), looking for blue cells, using the 
dissecting scope 
Make observations in your lab notebook 7) Label 3 microfuge tubes to align with each sample. 8) Transfer each brine shrimp from the slide into the corresponding microfuge tube 9) Add 50 μl of PBS to the tube and use the mechanical disruption tool to homogenize the 
sample for ~2 minutes until a cell suspension has been generated. 10) Load 30-50 μl of your sample onto a new glass slide and add a cover slip. 11)Observe the sample on high power and use the scale below to qualitatively assess cytotoxicity” 0-no blue 1-some blue 2-fairly blue 3-totally blue.

Weekly leaderboard

Home
Homework Help 3,900,000
Biology 460,000
Start filling in the gaps now
Log in