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The green fluorescent protein gene is either:

An endogenous gene that naturally exists in the drosophila genome

OR

Often exists as a transgender in the drosophila genome, flies that bear a GFP gene is considered as transgenic flies

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Jean Keeling
Jean KeelingLv2
29 Sep 2019

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Flies with a dominant mutation in a particular gene have an additional set of wings. Through mapping techniques, you identify a candidate gene, gene X, on an autosomal chromosome that you think results in extra wings when mutated. When you sequence gene X in the mutant fly with extra wings, you notice a missense mutation in the middle of the coding region of gene X. What is the best way to verify that the mutation in gene X is responsible for the dominant phenotype?

a. Starting with a wild-type fly, knockout gene X to generate a null allele and see if flies that are heterozygous or homozygous for the null allele have extra wings.

b. Starting with the mutant fly that is heterozygous for the dominant allele, insert a wild type copy of gene X (complete with its own promoter) elsewhere in the genome of the fly and see if it rescues the mutant and results in normal/wild-type wings.

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You have a strain of flies that are homozygous for a deletion of 2 neighboring genes, A and B (genotype aabb). Wild type flies have transparent wings but these aabb flies have rainbow-colored wings. Which of the following experiments would provide you with more information about which of these genes (if either) is responsible for the rainbow-colored wings?

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Kruppel and giant are 2 gap genes in drosophila for which the maternal effect gene bicoid serves as a transcriptional activator. Bicoid protein is present in an concentration gradient in the drosophila embryo, with the highest levels at the anterior end and progressively lower levels as one moves toward the posterior pole.

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