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29 Sep 2019
Protein Name Molecular weight (daltons pI Alpha-antitrypsin 45,000 5.4 Cytochrome c 13,400 10.6 Myoglobin 17,000 7.0 Serum albumin 69,000 4.8 Transferrin 90,000 5.9
1. The following five proteins, which are listed with theirmolecular weights and isoelectric points, were separated bySDS-PAGE. Give the order of their migration from the top to thebottom of the gel.
2. If the five proteins listed above were separated in anisoelectric- focusing experiment, what would be the order of theirdistribution between the positive (+) and negative (-) ends of thegel?
| Protein Name | Molecular weight (daltons | pI |
| Alpha-antitrypsin | 45,000 | 5.4 |
| Cytochrome c | 13,400 | 10.6 |
| Myoglobin | 17,000 | 7.0 |
| Serum albumin | 69,000 | 4.8 |
| Transferrin | 90,000 | 5.9 |
1. The following five proteins, which are listed with theirmolecular weights and isoelectric points, were separated bySDS-PAGE. Give the order of their migration from the top to thebottom of the gel.
2. If the five proteins listed above were separated in anisoelectric- focusing experiment, what would be the order of theirdistribution between the positive (+) and negative (-) ends of thegel?
Reid WolffLv2
29 Sep 2019
