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Be able to explain the experiments and evidence that Griffiths,and then Avery MaCleod and McCarthy used to discover that DNA isthe genetic material

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Jean Keeling
Jean KeelingLv2
29 Sep 2019

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Related questions

Explain the experiments of Griffiths (1928) and Hershey Chase (1952) and discuss the evidence that these experiments provided indicating that DNA is the genetic material in bacteria and viruses.

Please answer all the questions

Define

1. Chargaff’s rule –

2. Semiconservative replication –

Fill in the Blank

3. Adjacent nucleotides are held together by a covalent bond called a _____________________ bond.

4. ________________________________ is the enzyme the adds nucleotides to the free 3’ hydroxyl group on a growing stand of DNA.

True or False

5. Hershey and Chase determined that DNA was the material of heredity when radiolabeled sulfur from a virus was injected in to an E. coli cell.

6. After coinfecting E. coli with “heavy” and “light” viral DNA, Meselson and Weigle observed a range of densities in the CsCl gradient which was evidence for recombination between the two stands of DNA.

Short Answer

7. Describe what happens when DNA replicates making sure to include the enzymes involved in the process.

8. Describe the experiments by Griffith, Avery, MacLeod, and McCarty that provided evidence for transformation and identified the transforming principle?

An important trait that some bacteria have is resistance to tetracycline (a broad-spectrum antibiotic). Other bacterial strains are inhibited by tetracycline (they cannot reproduce and form colonies in its presence). Your challenge: diagram and briefly describe an experiment to test the hypothesis that the tetracycline resistance is a trait that is inherited and encoded by DNA. Hints: look to the experiments of Griffith and Avery, MacLeod, and McCarty for guidance; ideas for materials: Tetracycline-resistant bacteria culture (Tcr cells), tetracycline-sensitive bacteria culture (Tcs cells), agar plates with tetracycline, test tubes, boiling water.

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