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29 Sep 2019
You cut pUC18 vector DNAwith SmaI. You cut the insert DNA with EcoRI. Can you clone theinsert into this vector?
No
Yes, without any further modification of the insert.
Yes, only after making the insert DNA blunt-ended by anenzyme.
Yes, only after making the vector DNA sticky using anexonuclease.
You need to separate ssDNA molecules that are differentfrom each other by one or a few nucleotides in size. You shoulduse________ gel.
1-2% native agarose
2-3% denaturing agarose
3-4% native polyacrylamide
5-6% denaturing polyacrylamide gel
2% agarose pulsed field
You cut pUC18 vector DNAwith SmaI. You cut the insert DNA with EcoRI. Can you clone theinsert into this vector?
| No |
| Yes, without any further modification of the insert. |
| Yes, only after making the insert DNA blunt-ended by anenzyme. |
| Yes, only after making the vector DNA sticky using anexonuclease. You need to separate ssDNA molecules that are differentfrom each other by one or a few nucleotides in size. You shoulduse________ gel.
|
Hubert KochLv2
29 Sep 2019
